电化学活化玻碳电极上葡萄糖氧化酶的吸附与直接电化学  

THE ADSORPTION AND DIRECT ELECTROCHEMISTRY OF GLUCOSE OWIDASE ON ELECTROCHEMICALLY ACTIVATED GLASSY CARBON ELECTRODE

作　　者：戴鸿平[1] 吴辉煌[1] 周绍民[1] 

机构地区：[1]厦门大学化学系,厦门361005

出　　处：《化学研究与应用》1991年第3期47-52,共6页Chemical Research and Application

基　　金：国家教委博士点基金

摘　　要：利用循环伏安法和SNIFTIRS法研究了葡萄糖氧化酶(GOD)在经电化学活化的玻碳(GC)电极上的吸附与直接电化学行为。GC电极经活化后对GOD的吸附大为增强。吸附速度与吸附量同GC电极活化时高电位氧化时间、GOD浓度、溶液pH值及通氮搅拌有关。SNIFTIRS实验表明,GOD可能主要吸附于活化新生石墨结构晶棱或晶面上。表面微晶石墨结构可能为吸附GOD同GC电报的直接电子传递场所。Cyclic voltammetry and in-situ SNIFTIRS were used to study the adsorption and direct electr ochemistry of Glucose Olucose Oxidase (GOD) on electrochemically activated glassy carbin (GC) elecrode. Activation of GC enhancad strongly its ability to adsorb GOD. The adsorption depended on the anodization time during activation, the conentration of GOD, the pH of the solution and the agitating by bubbling N_2. In-situ SNIFTIRS spectra implied that the GOD might be mainly adsorbed on the new produced graphite micro-crystals.

关 键 词：葡萄糖氧化酶 吸附 直接电化学 玻碳电极 SNIFTIRS 

分 类 号：O6[理学—化学]