微载体系统Vero细胞培养日本乙型脑炎病毒的电镜观察  

Electron Microscopic Observations on Japanese Encephalitis Virus (JEV) Grown in Vero Cell in Microcarrier System

作　　者：王雅[1] 陆秀英[1] 向诚 金凤 贺适民 宋家骊[1] 陈列胜[1] 华平 

机构地区：[1]卫生部上海生物制品研究所,上海200052

出　　处：《中国生物制品学杂志》1991年第1期40-43,共4页Chinese Journal of Biologicals

摘　　要：本文通过扫描和透射电镜分别可观察到微载体系统表面的Vero细胞及其内部的日本乙型脑炎病毒的形态,病毒的装配繁殖部位可能在线粒体,通过内质网形成有包膜的完整病毒颗粒,病毒大小为30～46nm。感染病毒后2～3天为繁殖高峰。本试验工作为乙型脑炎病毒在Vero细胞及微载体系统中的繁殖提供了形态学证据。The JEV multiplying in Vero cells in microcarrier system was studied by scanning and transmission electron microscopy. The virus was found in mitochondrion and endoplasmic reticulum. The size of the virus ranged from 30 to 46nm. The most abundant virus particles appeared 2～3 days after inoculation of virus.This is the morphological evidence showing that the JEV can multiply in the Vero cells in the microcarrier system.

关 键 词：微载体 VERO细胞 线粒体 

分 类 号：R977[医药卫生—药品]