氮氧自由基化合物对L7712细胞DNA RNA和蛋白质合成的抑制作用  

INHIBITION OF NITROXY RADICAL COMPOUNDS ON THE SYNTHESIS OF DNA, RNA AND PROTEIN OF LEUKEMIA 7712 CELLS IN VITRO

作　　者：刘力生[1] 郑荣梁[2] 宋勤[2] 顾继杰[2] 张自义[3] 魏陆林 

机构地区：[1]仲恺农业技术学院农业生物技术研究室 [2]兰州大学生物物理研究室 [3]兰州大学化学系

出　　处：《仲恺农业工程学院学报》1991年第1期30-37,共8页Journal of Zhongkai University of Agriculture and Engineering

摘　　要：2,2,6,6—四甲基哌啶氮氧自由基等自由基化合物,均比其还原体化合物对癌细胞DNA合成的抑制率高。四甲基哌啶氮氧自由基和四甲基吡咯啉氮氧自由基对L7712细胞DNA,RNA和蛋白质合成的抑制作用,在3—24小时内均随作用时间延长而增高,它们对癌细胞DNA合成的抑制作用机理,似属干扰代谢型。Inhibition of 2. 2, 6, 6 - tetramethul - isothiocyano piperdine- 1 -oxyl et al nitroxy radical compounds on the synthesis of DNA in the leukemia 7712 cells in vitro was higher than that of its reduced compounds. The incorporations of [3H] thymidine, [3H] uridine and [3H] leucine into DNA, RNA, and protein respectively in leukemia 7712 cells were inhibited by the comprex of 2. 2, 6, 6 - tetramethy 1 - 4 - isothiocyano-piperidine-1- oxyl and 2, 2, 5, 5 - tetramethy 1 - 4-isocyano-pyrrolion - ocyl with praseodymium dicitrite 50 μg/ml for 3 - 24 hours. The degree of inhibition increased in proportion to the incubation time. After removing the complex from the medium entirely, the rate of DNA synthesis increased with time over 1-7 hours. This result indicated that the inhibition mechanism was likely metabolic lock agent for DNA synthesis.

关 键 词：氮氧自由基化合物 白血病L7712癌细胞 DNA RNA 蛋白质 

分 类 号：S[农业科学]