补中益气汤对A549荷瘤小鼠脾脏上Caspase-3蛋白表达影响研究  

Effect of Buzhong Yiqi Decoction on the expression of Caspase-3 protein of A549 tumor-bearing mice'spleen

作　　者：王雨[1] 刘春英[1] 

机构地区：[1]辽宁中医药大学研究生院,辽宁沈阳110032

出　　处：《中国生化药物杂志》2014年第1期19-21,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：辽宁省自然基金资助项目(20102145)

摘　　要：目的研究补中益气汤对半胱氨酸天冬氨酸酶—3(Caspase—3)在人肺腺癌细胞株(A 549)荷瘤小鼠脾脏上表达的影响。方法随机对照法将24只A 549荷瘤BALB/c小鼠分为空白对照组,补中益气汤低剂量组、补中益气汤高剂量组,顺氯氨铂(cisplatin,DDP)组,补中益气汤每日给药2次连续10天,顺铂每日给药1次连续3天,在连续15天后将所有荷瘤小鼠的脾脏取出。将新鲜的脾脏组织固定后制作石蜡切片。免疫组织化学法检测各组脾组织中Caspase—3蛋白表达的部位与含量多少,聚合酶链式反应(polymerase chain reaction,PCR)检测各组组织中Caspase—3 mRNA的表达。结果免疫组化结果显示与空白对照组相比,补中益气汤低剂量组Caspase—3蛋自的表达量显著增多(P<0.05),补中益气汤高剂量组蛋白的表达量增多(P<0.05)。而DDP组与空白对照组相比无统计学意义,DDP组与补中益气汤低剂量组、补中益气汤高剂量组相比的差异均有统计学意义(P<0.05,P<0.01)。RT—PCR结果同免疫组化结果具有一致性。结论补中益气汤可增加A 549荷瘤小鼠脾脏上Caspase—3蛋白的表达,从而使Caspase—3诱导肿瘤细胞凋亡的作用增强。Objective To analyses the effect of Buzhong Yiqi Decoction on the expression of Caspase-3 in human lung adenocarcinoma cells (A 549) tumor-bearing mice’spleen. Method 24 BALB/c mice were randomly divided into the control group(given saline),low-dose and high-dose Buzhong Yiqi Decoction groups,cisplatin(DDP)group;Buzhong Yiqi Decoction was administered twice daily for 10 days consecutively and DDP was administered once daily for 3 days consecutively, after 15 days was the spleens of all tumor-bearing mice were obtained.The expression of Caspase-3 protein and Caspase-3 mRNA in every group were detected by immunohistochemical method seperately and polymerase chain reaction method seperately. Results Compared with control group,the result of immunohistochemistry showed that the expression of the Caspase-3 protein in Buzhong Yiqi Decoction low-dose group and high-dose group were both ssignificantly increased (P<0.05). The differences hetween DDP group and control group was not significant, but the differences between DDP sroup and Buzhong Yigi Decoction Low-dose sroup and hishdoe group were significant (P<0.05, P<0.01).The result of RFPCR was accordance with the result of immunohisto chemistry. Conclusion Buzhong Yiqi Decoction can increase the expression of Caspase-3 protein in A549 ctumor-bearing mice’spleen,and promote the Caspase-3’s inducing effect on tumor cell apoptosis.

关 键 词：人肺腺癌细胞株 半胱氨酸天冬氨酸酶-3 补中益气汤 脾脏 

分 类 号：R285.5[医药卫生—中药学]