卡托普利预处理对急性心肌损伤大鼠细胞凋亡及gp 130表达的影响  

Effect of apoptosis and gp 130 expression by Cap preconditioning on rats of acute myocardial injured

作　　者：顾明[1] 荆雪冰[1] 车兆梅[1] 刘同库[1] 周艳[2] 

机构地区：[1]北华大学附属医院心内科,吉林吉林132011 [2]北华大学基础医学院组胚教研室,吉林吉林132013

出　　处：《中国生化药物杂志》2014年第1期43-45,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的研究卡托普利预处理对急性心肌损伤大鼠心肌细胞凋亡及gp 130表达的影响及意义。方法将60只Wistar雄性大白鼠随机分为3组:异丙肾上腺素损伤组、卡托普利预处理组、对照组(n=20);用TUNEL方法检测心肌细胞凋亡;用免疫组化方法检测Bcl-2和Bax蛋白表达;用Western blot方法检测心肌组织gP 130表达。结果卡托普利预处理组与异丙肾上腺素损伤组比较,心肌细胞凋亡指数降低,Bax、gP130表达减少,Bcl-2表达增加(P<0.01或P<0.05)。结论卡托普利预处理可通过降低gP 130的表达来减少急性心肌损伤引起的细胞凋亡。Objective To study the influence of Captopril preconditioning on apoptosis and the expression of gp 130 in rats of acute myocardial injured. Method Wistar rats were randomly divided into three groups:control group, Iso group and Cap preconditioning group. Cardiomyocytes apoptosis was detected with TUNEL method. The expressions of Bcl-2 and Bax proteins in myocardium were tested by immunohistochemistry. The expression of gp 130 was tested with Western blot method. Results The index of cardiomyocytes apoptosis was decreased, the expressions of Bax proteins and gp 130 were decreased and the expression of Bcl-2 proteins was increased in Cap preconditioning group compared with Iso group (P<0.01 or P<0.05). Conclusion The expression of gp 130 could be decreased through Cap preconditioning, which can reduce the cardiomyocytes apoptosis.

关 键 词：卡托普利 异丙肾上腺素 糖蛋白130 细胞凋亡 

分 类 号：R541.4[医药卫生—心血管疾病]