依托咪酯对胰岛素抵抗大鼠胸主动脉的舒血管作用研究  

The vasodilating effects induced by etomidate in aortas of insulin resistant rats

作　　者：薛文鑫 李静 

机构地区：[1]煤炭总医院药学部,北京100028

出　　处：《中国生化药物杂志》2014年第2期51-54,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的：研究依托咪酯对胰岛素抵抗（insulin resistance，IR）大鼠胸主动脉舒血管作用的可能机制。方法记录苯肾上腺素预收缩的离体大鼠主动脉环张力变化，观察依托咪酯的舒血管作用及不同工具药对其作用的影响。结果依托咪酯（10-6～10-4 mol·L-1）对正常大鼠和IR大鼠血管环均有内皮依赖的、浓度依赖的舒张作用。在内皮完整的血管环，左旋硝基精氨酸甲酯（L-NAME，0.1 mmol·L-1）能明显抑制2组大鼠依托咪酯的血管舒张作用，而且L-NAME对IR大鼠舒血管作用的抑制程度显著高于正常大鼠（P<0.05）。吲哚美辛（10μmol·L-1）对IR大鼠依托咪酯的血管舒张作用无明显影响。K+通道阻断剂格列本脲（10μmol·L-1）能明显抑制依托咪酯对IR大鼠的舒血管作用，而4-氨基吡啶（1 mmol·L-1），Iberiotoxin（100 nmol·L-1）和BaCl 2（1 mmol·L-1）无影响。结论依托咪酯可引起大鼠胸主动脉的舒张，IR增强了依托咪酯的舒张作用。依托咪酯对IR大鼠主动脉的舒张作用是内皮依赖性的，此作用可能和ATP敏感钾通道和NO的参与有关。Objection To investigate the effects of etomidate on aorta isolated from IR rats, and explore its underlying endothelium-related mechanism(s). Methods The IR animal model was made by feeding rats with high fructose diet for 8 weeks. Aortic rings were isolated and suspended in a tissue bath, and tensions were recorded isometrically. The effects of etomidate on provoked contractions of the rings were assessed in absence or presence of potassium channel blockers or NO-synthase inhibitors. Results Etomidate-induced relaxation in IR rings was greater than NC rings. NG-nitro-L-arginine methyl ester (L-NAME) or glibenclamide (Gli) inhibit significantly etomidate-induced relaxation in IR rings, and the inhibition of Gli was disappeared in endothelial-denuded aortic rings. Conclusion Etomidate cause vasodilation in IR rat aortas by an endothelial-dependent and independent manner. Impaired NO- and KATP channel-mediated relaxation and etomidate-induced increased availability of may involve in endothelial-dependent relaxation of etomidate in IR rat aortas.

关 键 词：胰岛素抵抗 依托咪酯 主动脉 内皮功能 钾通道 

分 类 号：R965[医药卫生—药理学]