小牛脾提取物注射液对免疫功能低下小鼠的免疫调节作用  

Immunomodulatory effect of calf spleen extractive injection on immunocompromised mice

作　　者：孙燕[1] 王冬梅[1] 

机构地区：[1]山东莱芜职业技术学院生物制药教研室,山东莱芜271100

出　　处：《中国生化药物杂志》2014年第2期58-60,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的研究小牛脾提取物注射液(calf spleen extractive injection,CSEI)对免疫功能低下小鼠免疫功能的影响。方法取BALB/c小鼠120只,按照随机数字表分为正常组(n=20)、模型组(n=20)、阳性对照组(胸腺肽,n=20)、CSEI高剂量组(1.20mL/kg,n=20)、CSEI中剂量组(0.60mL/kg,n=20)、CSEI低剂量组(0.30mL/kg,n=20)。除正常组外,第1、2、3天腹腔注射环磷酰胺(80mg/kg)造成免疫功能低下模型,连续给药10d后,以脏器指数、吞噬指数、自然杀伤细胞(natural killer cell,NK)杀伤率、外周血白细胞(white blood cell,WBC)计数、淋巴细胞(lymphocytes,LYM)计数及血清抗羊红细胞(sheep red blood cell,SRBC)IgG水平为考察指标,验证CSEI对免疫功能低下小鼠免疫功能的调节作用。结果与模型组相比,高、中、低CSEI组均能显著提高能够免疫功能低下小鼠脏器指数、吞噬指数、NK细胞杀伤活性、血清特异抗SRBC的IgG水平以及升高外周血白细胞及淋巴细胞计数(P<0.05)。结论CSEI对免疫功能低下小鼠免疫功能具有增强作用。Objective To investigate the effect of Calf Spleen Extractive Injection(CSEI) on immune function of immunocompromised mice. Methods BALB/C mice were divided into normal group (n=20), model group (n=20) and positive control group (n=20), CSEI high dose group (1.20 mL/kg, n=20), middle dose group (0.60 mL/kg, n=20) and low-dose group (0.30 mL/kg, n=20). Besides the normal group, mice in other groups were intraperitoneal injected cyclophosphamide at dose of 80 mg/kg for three consecutive days to induce immunocompromised model. Continuous dosing after 10 days to viscera index, index, natural killer cells (natural killer cell, NK) kill rate, the number of peripheral blood leukocytes (white blood cell, the WBC), lymphocyte count (lymphocytes, LYM) and serum against sheep red blood cells (the Sheep Red blood cell, SRBC) IgG levels as examining index, verify the CSEI effect on the regulation of immune function was low immune function in mice. Results Compared with model group, CSEI groups of high middle and low dose had improved viscera index, phagocytic coefficient, NK cell killing activity, serum specific antibody level, peripheral WBC count and lymphocyte count(P<0.05). Conclusion CSEI has possess immunomodulatory effects on the immunocompromised mice.

关 键 词：小牛脾提取物 注射液 免疫功能低下 免疫调节 

分 类 号：R967[医药卫生—药理学]