内皮祖细胞移植前后兔肺动脉高压模型血浆血栓素A2和前列腺素I2的水平变化  

The change of TXA2 and PGI2 in Rabbit model of pulmonary hypertension after progenitor cell transplantation

作　　者：杨志瑜[1] 夏岳[1] 戚国庆[1] 

机构地区：[1]河北医科大学第一医院心内科,河北石家庄050017

出　　处：《中国生化药物杂志》2014年第3期30-33,36,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的探讨移植内皮祖细胞(endothelial progenitor cells,EPCs)后,野百合碱(monocrotaline,MCT)诱导兔肺动脉高压(pulmonary artery hypertension,PHA)模型中血浆血栓素A2(thromboxane A2,TXA2)、前列腺素I2(prostaglandin I2,PGI2)水平的变化。方法建立野百合碱诱导兔肺动脉高压模型;体外分离诱导兔内皮祖细胞,并进行鉴定;将50只新西兰大白兔随机分为3组,正常对照组和模型组经尾静脉注射M199培养基,EPCs治疗组经尾静脉注射荧光标记的EPCs。3周后测定兔血浆血栓素B2(thromboxane B2,TXB2)和6-酮-前列腺素1α(6-keto-prostagland in F1 alpha,6-keto-PGF1α)的含量,并计算2者的比值。结果与模型组相比,EPCs治疗组血浆中6-keto-PGFlα的含量显著增加,为(130.67±17.54)μmol/L;TXB2的含量显著降低,为(402.89±27.98)μmol/L;2组TXB2/6-keto-PGF1α的比值差异有统计学意义(P<0.01)。结论移植内皮祖细胞可以降低由野百合碱诱导的肺动脉高压中TXA2的含量及增加PGI2的含量,从而逆转肺动脉高压的损伤。Objective To discuss the changes of plasma TXA2 and PGI2 levels in the rabbit model of pulmonary hypertension (PHA)induced by monocrotaline (MCT)when transplanted with endothelial progenitor cells (EPCs). Methods The rabbit pulmonary hypertension model was constructed by MCT induction. Rabbit endothelial progenitor cells were induced,separated and identified in vitro.50 New Zealand white rabbits were randomly divided into 3 groups,normal control group and model group were given M199 culture medium by tail vein injection,EPCs treatment group were given EPCs marked with fluorescent though tail vein injection. The levels of plasma TXB2 and 6-keto-PGF1αwere detected and their ratio were calculated after three weeks. Results Compared with model group,the content of 6-keto-PGFlαin plasma in EPCs group was (130.67 ±17.54)u mol/L,which was increased sinnificantly. The content of TXB2 was (402.89 ±27.98 )u mol/L,which was decreased significantly.The ratio of TXB2 to 6-keto-PGF1 alpha between two groups was statistically significant(P<0.01 ). Conclusion EPCs transplantation can reduce the content of TXA2 and increase the content of PGI2 in the pulmonary hypertension rabbits induced by monocrotaline,then reverse the damage of pulmonary hypertension.

关 键 词：内皮祖细胞 肺动脉高压 TXA2 PGI2 

分 类 号：R453.9[医药卫生—治疗学]