调气行水合剂对肝癌腹水小鼠模型细胞凋亡基因的影响  

Effect of the Tiao qi xing shui Decoxction on Bax,Caspase-3,TGF-β1 Gene expression in mouse with ascetes hepatoma

作　　者：杨志新[1] 尤建良[1] 浦琼华[1] 

机构地区：[1]无锡市中医医院肿瘤科,江苏无锡241071

出　　处：《中国生化药物杂志》2014年第3期64-67,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：医学科技发展基金无锡市医管中心科研项目(YGZ1010)

摘　　要：目的研究调气行水合剂结合顺铂、白介素-2腹腔注射对肝癌腹水小鼠模型细胞凋亡基因Bax、Caspase-3、TGF-β1蛋白表达的影响,从分子生物学水平揭示其治疗肝癌腹水的机制。方法将72只纯系昆明小鼠随机分为对照组、纯中药组、中西医结合大、中、小剂量组及西药组,每组12只。用药结束后,处死小鼠,应用免疫组化法检测小鼠肝肿瘤组织Bax、Caspase-3、TGF-β1蛋白表达情况。结果通过图像分析系统对免疫组化图像面积及积分光密度进行测算,中西医结合大剂量组细胞凋亡基因Bax蛋白的阳性表达率显著高于对照组(P<0.01)。中西医结合大、中剂量组细胞凋亡基因Caspase-3、TGF-β1蛋白的阳性表达率显著高于对照组(P<0.01)。结论中西医结合大剂量组可明显增加细胞凋亡基因Bax蛋白表达,中西医结合大、中剂量组可明显增加细胞凋亡基因Caspase-3、TGF-β1蛋白表达,诱导细胞凋亡。揭示了调气行水合剂联合顺铂、白介素-2腹腔注射治疗肝癌腹水的作用机制。Objective To investgate the effect of Tiao qi xing shui decoction combined with peritoneal injection DDP and IL-2 on Bax,Caspase-3, TGF-β1 gene expression in mouse with ascetes hepatoma,and explore its mechanism from molecular biology.Methods 72 pure lines of Kunming mice were randomly divided into control group,Chinese medicine group,combination of traditional Chinese and Western medicine,in high,middle,low dose group and Western medicine group,each had 12.All mices were killed after treatment,and the expression of Bax,Caspase-3,GF-β1 protein in mouse liver tumor tissues were detected by Immunohistochemical. Results Immunohistochemical results showed that,the positive expression of apoptosis gene Bax protein in high dose group of TCMand Western Medicine was higher than that of control group(P<0.01),and the positive expression of apoptosis gene Caspase-3,TGF-β1 protein in high and middle dose group of TCMand Western Medicine were higher than that of control group(P<0.01).Conclusion The high dose group of TCM and Western Medicine can significantly increase the expression of Bax protein,the high and middle dose guoup of TCMand Western Mdicine can significantly increase the expression of Caspase-3,TGF-β1 protein,induction apoptosis.

关 键 词：肝癌腹水 调气行水合剂 BAX CASPASE-3 TGF-Β1 细胞凋亡 

分 类 号：R273[医药卫生—中西医结合]