紫外分光光度法测定链霉菌NG-715抗真菌组分的含量  

UV spectrophotometric determination of the antifungal component produced by Streptomyces NG-715

作　　者：袁敏[1] 罗建成[2] 侯俊然[1] 李晶[1] 陈志国[1] 

机构地区：[1]南阳理工学院医学实验中心,河南南阳473004 [2]南阳理工学院生物与化学工程学院,河南南阳473004

出　　处：《中国生化药物杂志》2014年第4期168-170,174,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：南阳理工学院青年基金项目(NG2009QNJJ10);河南省科技攻关计划项目(142102310508)

摘　　要：目的运用紫外分光光度法测定链霉菌NG-715抗真菌组分的含量。方法以链霉菌NG-715抗真菌组分为指标性成分,在339 nm波长处进行含量测定。结果紫外分光光度法测定链霉菌NG-715抗真菌组分浓度在8μg/mL^20μg/mL的范围内具有良好的线性关系,平均回收率为99.98%(n=6),RSD=0.11%。结论本法快速准确,重现性好,简便易行,可用于链霉菌NG-715抗真菌组分含量的测定。Objective To establish a method for the determination of the antifungal component produced by Streptomyces NG-715.Methods The antifungal component was used as the reference component for UV spectrophotometry detemined at 339 nm. Results By spectrophotometry,a good linearity was established within 8μg/mL^20 μg/mL,and the average recovery rate was 99.98%(n=6 )with RSD being 0.1 1%.Conclusion The method is simple,rapid,accurate with good reproducibility for determining the antifungal component produced by Streptomyces NG-715.

关 键 词：链霉菌NG-715 抗真菌组分 紫外分光光度法 

分 类 号：Q936[生物学—微生物学]