Antimicrobial activity against periodontopathogenic bacteria,antioxidant and cytotoxic effects of various extracts from endemic Thermopsis turcica  

作　　者：Elif Burcu Bali Leyla Acik Glin Akca Meral Sarper Mualla Pinar Eli Ferit Avcu Mecit Vural 

机构地区：[1]Health Services Vocational School.University of Gazi [2]Department of Biology,Faculty of Science.University of Gazi [3]Department of Medical Microbiology,Faculty of Dentistry,University of Gazi [4]Cancer and Stem Cell Research Center,Gulhane Military Medical School [5]Department of Hematology,Cancer and Stem Cell Research Center.Gulhane Military Medical School

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2014年第7期505-514,共10页亚太热带生物医学杂志（英文版）

基　　金：Supported by Minsitry of Devetopment of Tuckish Republie for Foundation of Moleculer Biology Research Center(Grant No.1998K121480)

摘　　要：Objective:To investigate the in vitro antimicrobial potential of Thermopsis turcica Kit Tan,Vural&Kckdk against periodoutopathogenie bacteria,its antioxidant activity and cytotoxic effect on various cancer cell lines.Methods:In vitro antimicrobial activities of elhanol.methanol,ethyl acetate(ElAc,n-hexane and water extracts of Thermopsis turcica herb against periodontopathogenic bacteria,Aggregatibacter actinnmycelemconilans ATCC 29523 and Porphyromonas gingivalis ATCC 33277 were tested by agar well diffusion,minimum inhibitory concentration(MIC)and minimal bactericidal concentration(MBC),Antioxidant properties of the extracts were evaluatod by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity and p-carotene bleaching methods.Amounts of phenolic contents of the extracts were also analysed by using the Folin-Ciocalteu reagent.Additionally,cytotoxic activity of the extracts on androgcn-insensilivc prostate cancer,androgen—sensitive prostate cancer,chronic myelogenous leukemia and acute promyelocytic leukemia bunian cancer cell lines were determined by 3-4,5-dimelhylthiazol-2-yh-2,5-diphenyltclrazolium bromide assay.Human gingival fibroblast cells were used as a control.Results:Our data showed that ELAc extract had the highest antimicrobial effect on Aggregatibacter actinomycetemitans(MIC:1.562 mg/ml_MHC:3.124 mg/ml.)and Porph yromonas gingiralis(MIC:0.781 mg/mL,MBC:1.562 mg/mL).In antioxidant assays.ElAc extract exhibited also the highest radical scavenging activity[IC_(50)=(30.0±0.3)μg/ml.]and the highest inhibition[(74.35±0.30)%]|against lineloic acide oxidation.The amount of phenolic content of it was also the highest[(l62.5±l.2)μg/mg gallic acid].In cytotoxic assay,only etbanol[IC_(50)=(80.00±1.21)μg/ml.]and EtAc extract[IC_(50)=(70.0±0.9)μg/ml]were toxic on acute promyeloeytic leukemia cells at 20—100μg/mL.P<0.05>.However,no toxic effect was observed on human gingival fibroblast cells.Cunclusions:According to our findings,owing to its antioxidant and cytotoxic potential,EtAc Objective:To investigate the in vitro antimicrobial potential of Thermopsis turcica Kit Tan,Vural&Kckdk against periodoutopathogenie bacteria,its antioxidant activity and cytotoxic effect on various cancer cell lines.Methods:In vitro antimicrobial activities of elhanol.methanol,ethyl acetate(ElAc,n-hexane and water extracts of Thermopsis turcica herb against periodontopathogenic bacteria,Aggregatibacter actinnmycelemconilans ATCC 29523 and Porphyromonas gingivalis ATCC 33277 were tested by agar well diffusion,minimum inhibitory concentration（MIC）and minimal bactericidal concentration（MBC）,Antioxidant properties of the extracts were evaluatod by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity and p-carotene bleaching methods.Amounts of phenolic contents of the extracts were also analysed by using the Folin-Ciocalteu reagent.Additionally,cytotoxic activity of the extracts on androgcn-insensilivc prostate cancer,androgen—sensitive prostate cancer,chronic myelogenous leukemia and acute promyelocytic leukemia bunian cancer cell lines were determined by 3-4,5-dimelhylthiazol-2-yh-2,5-diphenyltclrazolium bromide assay.Human gingival fibroblast cells were used as a control.Results:Our data showed that ELAc extract had the highest antimicrobial effect on Aggregatibacter actinomycetemitans（MIC:1.562 mg/mlMHC:3.124 mg/ml.）and Porph yromonas gingiralis（MIC:0.781 mg/mL,MBC:1.562 mg/mL）.In antioxidant assays.ElAc extract exhibited also the highest radical scavenging activity[IC50=（30.0±0.3）μg/ml.]and the highest inhibition[（74.35±0.30）%]|against lineloic acide oxidation.The amount of phenolic content of it was also the highest[（l62.5±l.2）μg/mg gallic acid].In cytotoxic assay,only etbanol[IC50=（80.00±1.21）μg/ml.]and EtAc extract[IC50=（70.0±0.9）μg/ml]were toxic on acute promyeloeytic leukemia cells at 20—100μg/mL.P&lt;0.05&gt;.However,no toxic effect was observed on human gingival fibroblast cells.Cunclusions:According to our findings,owing to its antioxida

关 键 词：THERMOPSIS turcica Antimicrobial activity Periodontopathogenic BACTERIA ANTIOXIDANT EFFECT Phenolic content Cytotoxic EFFECT Human GINGIVAL fibroblast Acute PROMYELOCYTIC leukemia 

分 类 号：R285[医药卫生—中药学]