一种新型仿生抗菌剂的功能研究  

A Study on a Novel Biomimetic Antimicrobial Agent

作　　者：赵彪[1] 高杨[1] 周华锋[1,2] 段明星[1] 

机构地区：[1]清华大学生命科学学院生物膜与膜生物工程国家重点实验室,北京100084 [2]江苏隆力奇生物科技股份有限公司,江苏215555

出　　处：《中国生物工程杂志》2013年第7期31-35,共5页China Biotechnology

摘　　要：抗菌剂α-炔丙基-γ-亚甲基-γ-丁内酯是以天然抗菌物质原白头翁素为基础,利用仿生技术合成出的新型抗菌剂。通过悬液杀菌法进行最小抑菌浓度测定、悬液定量法及活体菌落计数法进行最小杀菌浓度测定等生化试验对其抗菌效果进行了检验;通过细胞壁结构的完整性实验及透射电镜观察等理化实验对其抗菌机理做初步研究,为其应用于实践提供依据。抗菌剂α-炔丙基-γ-亚甲基-γ-丁内酯对大肠杆菌、金黄色葡萄球菌、白色念珠菌及马拉色菌均具有良好的抑菌作用,对马拉色菌的抑菌作用尤为突出,并对其具有良好的杀菌作用。其对马拉色菌的最小抑菌浓度为0.5mg/ml,当其浓度在10mg/ml时,对马拉色菌具有瞬杀作用(作用1min对球形马拉色菌的杀灭率可达到99.99%)。通过胞壁完整性实验以及电镜观察实验表明该抗菌剂的抑菌机制并不是完全破坏胞壁结构,但是对细胞壁有一定影响。Using biomimetic synthesis technology,we obtained a novel antimicrobial agent α-propargyl-γmethylene-γ- butyrolactone on the basis of protoanemonin. We characterized bacteriostatic effect and antimicrobial mechanism of the agent using physical and chemical analysis. We carried out the minimum inhibitory concentration (MIC) determination,suspension quantitative germicidal test,and other tests to verify its antimicrobial effect,the mechanism of which were primarily studied through cell wall integrity test and transmission electron microscopy. We found that the novel agent α-propargyl-γ-methylene-γ-butyrolactone had a good inhibitory effect on various bacterial species,especially on Malassezia globosa. Its MIC to malassezia was 0. 5mg/ml. Our suspension quantitative germicidal test,using Sodium thiosulfate as neutralizer,showed a high antibacterial efficiency that up to 99. 99% of Malassezia globosa is killed after exposed to 10mg/ml agent for only 1min. Membrane integrity experiments and transmission electron experiment suggested that the antimicrobial effect is not due to destroying the cell wall systerm,but it may have some impact on the cell wall.

关 键 词：仿生合成 球形马拉色菌 抑菌作用 抑菌机制 

分 类 号：R944[医药卫生—药剂学]