蚕豆染色病毒RNA2全序列测定  

Complete sequence of the genomic RNA2 of Broad bean stain virus

作　　者：黄峰[1] 陈青[1] 谢毅璇 陈细红[1] 陈红运[1] 林石明[1] 

机构地区：[1]厦门出入境检验检疫局,福建厦门361026 [2]厦门市植保植检站

出　　处：《植物检疫》2013年第5期41-44,共4页Plant Quarantine

摘　　要：采用RT-PCR扩增并测定蚕豆染色病毒(Broad bean stain virus,BBSV)RNA2的基因组序列。BBSV RNA2基因组全长3478 nt,5'非编码区和3'非编码区分别为268 nt和237 nt。RNA2编码的多聚蛋白经切割后产生辅助蛋白(CR)、移动蛋白(MP)、大外壳蛋白(CP-L)和小外壳蛋白(CP-S)。基于多聚蛋白P2氨基酸序列构建的系统树显示:侵染豆科作物的Comovirus病毒构成1个分支,表明Comovirus病毒之间的亲缘关系具有寄主相关性。The genomic RNA2 of Broad bean stain virus(BBSV) is 3478 nt long excluding the poly(A).The 5' NTR of BBSV RNA2 consists of 268 nt,and the 3' NTR is 237 nt long.The polyproteins encoded by RNA2 are processed into cofactor protein required for RNA2 replication,movement protein,large coat protein and small coat protein.Phylogenetic tree based on the amino acid sequences of the polyprotein P2 revealed the host correlation because legume-infecting comoviruses constitute a single branch.

关 键 词：蚕豆染色病毒 豇豆花叶病毒属 RNA2 

分 类 号：S432.41[农业科学—植物病理学] S41[农业科学—农业昆虫与害虫防治]