冷冻前平衡处理对人精子冻融后相关参数的影响  

Pre-freezing equilibration improves post-thaw human semen parameters

作　　者：王亚楠[1] 邹沙沙[1] 肖倩[1] 刘勇[1] 曹小蓉[1] 李铮[1] 胡洪亮[1] 

机构地区：[1]上海交通大学医学院附属仁济医院泌尿外科/上海市男科学研究所精子发育与遗传学实验室/上海市人类精子库,上海200001

出　　处：《中华男科学杂志》2013年第10期886-889,共4页National Journal of Andrology

基　　金：国家自然科学基金(30672146,30973069);浦江计划(08PJ1400);国家重点基础研究发展计划(2010CB945200,2011CB944504)~~

摘　　要：目的:研究冻前平衡处理对冷冻精子复苏率的影响,优化一步熏蒸法,以寻找人类精子冷冻的最优方法。方法:使用50例自愿供精者的正常精液标本,同1例供精者的精液一式3份,分别采用不平衡直接冷冻法、室温平衡10 min后冷冻与4℃平衡10 min后冷冻的方法进行处理。复苏后用计算机辅助精液分析(CASA)系统分析3组标本冷冻前后精子运动参数的变化,并检测各组标本精子的畸形率和存活率。结果:4℃平衡组的前向运动精子复苏率[(61.88±16.94)%]显著高于未平衡组[(48.61±16.44)%]和室温平衡组[(49.41±13.77)%](P<0.05),而室温平衡组与未平衡组前向运动精子复苏率则无显著性差异。3组标本经冷冻复苏后其精子畸形率和存活率无显著性差异。结论:冻前4℃平衡10 min的冷冻前处理方法操作简单,实验条件易控,对提高精子复苏率有重要意义。Objective: To investigate the effect of pre-freezing equilibration on the cryosurvival of human sperm and to optimize the protocol of direct fumigation for the freeze-thawing of human sperm. Methods: We collected 50 semen samples from healthy donors,each subjected to cryopreservation with 3 different methods: non-equilibration freezing( Group A),10-min equilibration at room temperature before freezing( Group B),and 10-min equilibration at 4 ℃ before freezing( Group C). We examined all the post-thaw semen samples by computer-assisted semen analysis for the sperm motility parameters,and detected the sperm vitality and deformity index( SDI). Results: The recovery rate of progressive sperm motility was( 61. 88 ± 16. 94) % in Group C,remarkably higher than in A( [48. 61 ± 16. 44]%) and B( [49. 41 ± 13. 77]%)( P < 0. 05),but with no significant difference between the latter two. And there were no significant differences in sperm vitality and SDI among the three groups. Conclusion: Ten-minute equilibration at 4 ℃ before freezing can evidently improve the progressive motility of sperm in addition to its advantages of easy operation and controllable experimental condition.

关 键 词：精液冷冻 平衡处理 精子参数 

分 类 号：R698.2[医药卫生—泌尿科学]