丹参酮ⅡA对雌性家兔LQT2模型心肌细胞动作电位的影响  

The Effect of TanshinoneⅡA on the Transmembrance Action Potential of Myocardial Cells in LQT2 Rabbit Models

作　　者：梁玲[1] 谢强[1] 李卫华[1] 曾智[2] 黄峥嵘[1] 李德龙[1] 

机构地区：[1]厦门大学附属第一医院心内科,厦门361003 [2]四川大学华西医院心内科,成都610041

出　　处：《四川大学学报（医学版）》2013年第5期744-746,共3页Journal of Sichuan University(Medical Sciences)

基　　金：福建省青年科研课题(No.2007-2-44)资助

摘　　要：目的观察丹参酮ⅡA对雌性家兔长QT2(long QT,LQT2)模型跨左室壁不同部位心肌细胞动作电位的影响。方法采用全细胞膜片钳技术记录雌性家兔LQT2模型左室壁内、中、外3层心肌细胞的动作电位(action potential,AP),观察10mg/mL丹参酮ⅡA对3层肌细胞动作电位复极达90%时程(APD90)和跨室壁复极离散度(transmural dispersion of repolarization,TDR)的影响。结果 LQT2模型家兔左室3层心肌细胞中,中膜APD90最长,其次为内膜,最短为外膜,灌注10mg/mL丹参酮ⅡA后能缩短LQT2模型的各层心肌细胞的APD90,同时降低TDR。结论 10mg/mL丹参酮ⅡA能有效缩短LQT2模型的各层心肌细胞动作电位,同时降低各层心肌细胞动作电位复极的差异性。Objective To determine the effect of tanshinoneⅡA on the transmembrance action potential of three layers of myocardial cells in LQT2 rabbit models.Methods Whole-cell patch clamp technique was used to record the action potential duration 90(APD90)and the transmural dispersion of repolarization(TDR)of the epicardium,mid-myocardium and endocardium in the LQT2 rabbits.Results Of the three layers of myocardial cells,the mid-myocardium had the longest APD90 and the epicardium had the shortest APD90.The APD90 of all three layers of myocardial cells were shortened with infusion of 10mg/mL tanshinoneⅡA.Their TDR values also reduced by the infusion.Conclusion TanshinoneⅡA shortens APD90and reduces TDR values.

关 键 词：跨膜动作电位 跨室壁复极离散度 丹参酮ⅡA LQT2模型 

分 类 号：R541.7[医药卫生—心血管疾病]