不同针刺法对快速老化痴呆小鼠学习记忆能力的影响  

Effects of different acupuncture methods on the learning and memory ability in senescence-accelerated mice

作　　者：刘楠[1] 樊小农[2] 张艳[1] 麻聪聪[1] 樊同涛[1] 常女子 王晓光[1] 王慧[1] 

机构地区：[1]天津中医药大学研究生院,300193 [2]天津中医药大学第一附属医院

出　　处：《中华针灸电子杂志》2013年第4期166-170,共5页Chinese Journal of Acupuncture and Moxibustion（Electronic Edition）

基　　金：天津市高等学校科技发展基金计划项目(20090206);天津市应用基础及前沿技术研究计划重点项目(11JCZDJC19800);"十二五"国家科技支撑计划2013BAI13B03;国家重点基础研究发展计划(973计划)赞助课题(2012CB518505;2010CB 530500);长江学者和创新团队发展计划资助

摘　　要：目的探讨针刺补虚与泻实法对快速老化痴呆小鼠(senescence accelerated mouseprone/8,SAMP8)学习记忆能力的影响。方法选取36只SAMP8老化鼠,随机分为模型组、补虚组和泻实组,补虚组选取双侧三阴交、足三里穴施捻转补法针刺,泻实组选取人中穴施雀啄手法、双侧内关穴施捻转泻法针刺,1次/d,共15 d;选取12只抗快速老化小鼠(senescence accelerated mouseresistance/1,SAMR1)作为对照组。采用Morris水迷宫方法,连续记录5 d的逃避潜伏期、第1次穿越平台时间和游泳平均速度隐蔽平台实验数据符合球形检验,采用双因素方差分析统计,P<0.05为差异有统计学意义。结果经球形检验分析,5次重复测量的数据存在高度相关性(F=33.277,P<0.01),且组别和时间具有交互作用(F=2.943,P<0.01)逃避潜伏期:5 d来,SAMR1对照组显著缩短,而SAMP8模型组虽然也有变化,但改善不明显,两组分别由第1天的(62.19±17.66)s、(81.81±11.11)s(F=5.209,P<0.01)下降至第5天的(27.52±18.60)s、(72.63±17.09)s(F=10.993,P<0.01),差异有统计学意义(P<0.05);针刺补虚组与泻实组分别在第4和2天发挥疗效,而且均在第5天数值明显变化,分别为(47.34±24.60)s、(43.12±16.87)s,优于SAMP8模型组,且差异有统计学意义(P<0.05),虽未达到SAMR1对照组水平,但差异并无统计学意义(P>0.05)。第1次穿越平台时间:SAMP8模型组、SAMR1对照组第1和第5天的结果分别为(75.20±13.15)s、(50.17±19.17)s(F=5.559,P<0.01)和(59.88±19.11)s、(21.88±18.45)s(F=8.494,P<0.01),组间比较差异有统计学意义(P<0.05);针刺补虚组、针刺泻实组进行实验的前4 d改善不明显,第5天的结果分别为(34.05±22.72)s、(31.32±16.48)s,明显优于SAMP8模型组,且差异有统计学意义(P<0.05)。各组游泳平均速度比较差异无统计学意义。结论 SAMP8存在明显的学习记忆障碍,针刺补虚法与泻实法均可改善SAMP8小鼠的学习记忆能力,并具有针刺的蓄积性作用以及针刺方�Objective To investigate the effects of the SUPPLEMENT DEFICIENCY AND REDUCING EXCESS method of acupuncture on the learning and memory ability in senescence-accelerated mouse prone/8 (SAMP8). Methods A total of 36 SAMP8 mice were randomly divided into three groups including model group,SUPPLEMENT DEFICIENCY group and REDUCING EXCESS group. The bilateral sides of SP6 and ST36 were selected for twirling supplementation needling method in SUPPLEMENT DEFICIENCY group,once a day for 15 days. The DU26 was selected with bird-pecking needling method and bilateral PC6 were selected with twirling draining needling method in REDUCING EXCESS group. Twelve SAMP8 mice were selected as control group. Morris water maze was used to perform the learning and memory ability in three groups of mice. The two-way ANOVA test was used to compare the data in this study. A value of P<0.05 was considered statistically significant. Results The analysis of sphericity test showed that there was a high correlation between 5 times of repeated measures data (F=33.277,P<0.01), and there was a time-by-group interation (F =2.943,P <0.01). There was significantly reduced escape latency in control group (62.19±17.66)s and (27.52±18.60)s,and there was a reduce in model group (81.81±11.11)s and(72.63±17.09)s but there was no significance(P<0.01). The therapeutic effectiveness was found on the day-4 in SUPPLEMENT DEFICIENCY group and on the day-2 in REDUCING EXCESS group,which was the most significant on the day-5 (47.34±24.60)s and (43.12±16.87)s compared with that of model group (P<0.05). There was no significant difference in the therapeutic effectiveness between SUPPLEMENT DEFICIENCY and REDUCING EXCESS groups and control group (P>0.05). There were significant differences in escape latencies on day-1 and day-5 between model group (75.20±13.15)s and (50.17±19.17)s,(F=5.559,P<0.01)and control group (59.88±19.1)s and (21.88±18.45)s,(F=8.494,P< 0.01). There was no significant improvement in escape latency on the day-4 in SUPPLEMENT DEFICIENCY g

关 键 词：阿尔茨海默病 针刺 动物实验 SAMP8 

分 类 号：R245[医药卫生—针灸推拿学]