时间分辨荧光免疫技术在乙肝病毒血清学检测中的研究  

THE STUDY OF TIME-RESOLVED FLUO RESCENCE IMMUNOUSSAY IN DECECTION OF SENCM MARKERS OF HEPATITIS B VIRUS

作　　者：王泽民[1] 

机构地区：[1]内蒙古医科大学附属医院感染科,内蒙古呼和浩特010050

出　　处：《内蒙古医科大学学报》2013年第6期455-458,共4页Journal of Inner Mongolia Medical University

摘　　要：目的:探讨时间分辨荧光免疫分析(TRFIA)检测乙肝病毒血清标志物的临床价值。方法:用酶联免疫吸附试验(ELISA)和时间分辨荧光免疫分析对185例血清标本进行乙肝标志物的定性、定量检测和比较,对结果进行分析。结果:TRFIA检测乙肝五项指标具有良好的准确性和重复性。与ELISA相比,TRFIA在HB s Ag、抗-HB s、HB e Ag、抗-HB e的测定上差异无统计学意义(P>0.05),而在抗-HB c的测定上差异有统计学意义(P<0.05)。高、中浓度标本两种方法检测HB s Ag阳性均为10例,而在低浓度中,TRFIA阳性率要高于ELISA。经两种方法检测,测得9种模式,其中6种模式两着检出的阳性例数接近,3种模式(4-5、5、全阴)差异较大。结论:TRFIA法定量检测乙肝标志物的灵敏度更高,特异性更好,并有助于对治疗效果作出有效的判断。Objective:To study the clinical application value of time-resolved fluorescence immunoassay( TRFIA) for detection of serum markers of hepatitis B virus. Methods:Using enzyme im-munoassay( ELISA) and time-resolved fluorescence immunoassay respectively in 185 serum specimens, hepatitis B markers were qualitatively and quantitatively detected and compared,analysis of the results. Results:Five makers of hepatitis B was measured with good accuracy and repeatability by TRFIA. There was no remarkable significance between ELISA and TRFIA in detecting HBsAg、anti-HBs、HBeAg ang anti-HBe(P>0. 05). But there was significant difference between ELISA and TRFIA in detecting anti-HBc(P<0. 05). In each 10 samples of high and medium concentrations of HBsAg,all were positive by TRFIA and ELISA. However,the detection rate with TRFIA assay was higher than that with ELISA assay in 10 samples of low concentration. There were nine modes in two methods,positive example was dose to each other in six modes,there were difference in three modes. Conclusions Time-resolved fluorescence immunoassay for detection of serum markers of hepatitis B virus had good sensitivity and good specificity and it is helpful to estimate the effect.

关 键 词：时间分辨荧光免疫技术 酶联免疫吸附试验 乙肝病毒 

分 类 号：R446.61[医药卫生—诊断学]