五种短体线虫 DNA 条形码鉴定方法  

Identification of 5 Pratylenchus Species Utilizing DNA Barcoding Method

作　　者：魏亚东[1] 容万韬 赵立荣[3] 王金成[1] 黄国明[1] 郭京泽[1] 孙建华[2] 

机构地区：[1]天津出入境检验检疫局动植物与食品检测中心,天津300461 [2]天津师范大学,天津300387 [3]广东出入境检验检疫局,广东广州510623

出　　处：《华北农学报》2013年第6期136-139,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家质检总局项目(2011IK176;2011IK177);国家科技支撑计划项目(2012BAK11B03)

摘　　要：以穿刺短体线虫、咖啡短体线虫、伤残短体线虫、斯克里布纳短体线虫和落选短体线虫5种短体线虫的18个种群为试验材料,将其测序获得的ITS序列与GenBank中已提交的短体线虫序列进行了Blast比对和系统发育分析,以验证核糖体ITS序列作为DNA条形码的可行性。结果显示,核糖体ITS序列作为DNA条形码可以很好的用于鉴别以上5种短体线虫。The root lesion nematodes,Pratylenchus Flipjev,1936,are among the widespread and seriously de-structive plant endoparasites around the world .Due to the instability of morphological characteristics ,it was very dif-ficult to identify this kind of nematodes just by their morphology .In this study ,18 populations of the following 5 Pra-tylenchus species,i.e.Pratylenchus penetrans,P.coffeae,P.vulnus,P.scribneri and P.neglectus, were used as ex-periment materials ,and their ribosomal ITS sequences were obtained to conduct blast alignment and phylogenetic a -nalysis with other ribosomal ITS sequences of Pratylenchus species submitted in GenBank in order to verify the fea-sibility of ITS regions as DNA barcode .The results showed that DNA barcoding approach using ribosomal ITS re-gions as target fragment could be readily used for identification of the above-mentioned 5 Pratylenchus species .

关 键 词：短体线虫 ITS 系统发育 DNA条形码 

分 类 号：S433.89[农业科学—农业昆虫与害虫防治]