增强UV-B辐射对小麦叶肉细胞核内F-actin的影响  

Influence of enhanced UV-B radiation on F-actin of wheat mesophyll nucleus

作　　者：陈慧泽[1] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《广西植物》2013年第5期674-678,共5页Guihaia

基　　金：国家自然科学基金(30671061);山西省自然科学基金(200811059)

摘　　要：以增强紫外线B(UV-B)处理的小麦幼苗叶肉细胞核为材料,以异硫氰酸荧光素标记鬼笔环肽(FITC-Ph)荧光标记后,利用流式细胞仪以及激光共聚焦显微镜,对核内肌动蛋白丝(F-actin)进行研究。结果表明:小麦细胞核内确实存在F-actin;以FITC-Ph标记后用流式细胞仪检测显示,以10.08kJ·m-2·d-1增强的UV-B处理后的小麦叶片细胞核内F-actin的含量升高;激光共聚焦技术显示细胞核内Factin的分布,发现小麦叶肉细胞核核仁处F-actin含量较高。推测可能是增强UV-B辐射导致细胞质内Factin骨架断裂进入细胞核。究其具体作用还待进一步研究,该研究推测其与"分束分裂"有关。Nuclei which were treated after enhanced UV-B radiation were taken as materials in this research.The nucleus actin of wheat mesophyll was researched by fluorescent with FITC-ph and flow cytometry.The results showed that F-actin truly existed in the wheat mesophyll nucleus,which increased in its content after treated with the enhanced UV-B radiation.The FITC-ph fluorescent labeling displayed that the distribution and the content of the nucleus F-actin.We found that F-actin in the nucleolar was higher than the other areas in the wheat mesophyll nucleus.We speculated these were closely with'partition-bundle division'.

关 键 词：增强UV-B 小麦 原生质体 细胞核内F-actin 

分 类 号：Q942.4[生物学—植物学]