矮秆大豆突变体异黄酮含量变化的研究  

Isoflavone content change research of dwarf mutant soybean

作　　者：杨正坤[1] 龙施华[1] 周菲菲[1] 郝再彬[1] 王秀丽[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541006

出　　处：《广西植物》2013年第5期699-702,590,共5页Guihaia

基　　金：国家自然科学基金(31060193)

摘　　要：以大豆品种东农42及其两个矮秆突变体(HK8和HK11)为原料,采用Luminol-K3Fe(CN)6化学发光体系的流动注射化学发光法对茎、叶和种子中的异黄酮进行检测,同时与HPLC检测方法相比较。结果表明:该方法能较简便快速地检测异黄酮含量,相对标准偏差(RSD)为0.69%~2.30%,回收率为95.5%~104.5%。该实验准确地检测出三种大豆材料中异黄酮含量;异黄酮含量与株高成正比;种子的异黄酮含量比茎、叶的均高。The isoflavones of stems,leaves and seeds were detected by flow injection chemiluminescene method from the Dongnong 42and two dwarf mutants(HK8and HK11);at the same time,the flow injection chemiluminescence and HPLC were compared in this paper.The results of the study showed that the isoflavone content could be more simply and fleetly detected by flow injection chemiluminescene,the RSD was 0.69%-2.30%,and the recovery was 95.5%-104.5%.It can be concluded that the isoflavone can be accurately detected by flow injection chemiluminescene method in this experiment;the isoflavone is proportional to the plant height;the isoflavone content of seeds is higher than that of stems or leaves.

关 键 词：大豆异黄酮 含量 流动注射化学发光 株高 

分 类 号：Q946[生物学—植物学]