亳白芍与赤芍药材中芍药苷含量比较  

COMPARATIVE STUDY ON THE CONTENTS OF PAEONIFLORIN IN RADIX PAEONIAE ALBA AND RADIX PAEONIAE RUBRA

作　　者：秦亚东[1] 李飞[2] 金镭[3] 

机构地区：[1]安徽中医药高等专科学校,安徽芜湖241000 [2]安徽亳州市食品药品检验所,安徽亳州236800 [3]中国人民解放军第105医院,安徽合肥230031

出　　处：《南阳理工学院学报》2013年第3期125-128,共4页Journal of Nanyang Institute of Technology

基　　金：2013年度安徽省高校省级科研项目(KJ2013B114);安徽中医药高等专科学校校级自然科学基金(ZRKX1021)

摘　　要：目的:建立简单、快速、灵敏、准确的HPLC-DAD法测定亳白芍与赤芍药材中芍药苷的含量。方法:采用HPLC-DAD法,ZORBAX SB-C18(250 mm×4.6 mm I.D.,5μm)色谱柱;以乙腈为流动相A,0.10%磷酸水溶液为流动相B;等度洗脱;柱温25℃;检测波长为230 nm;流速为1.0 mL·min-1。分离度R大于1.5,理论塔板数N,按芍药苷计不低于4000。结果:亳白芍和赤芍药材中芍药苷的含量分别为24.43 mg·g-1、32.77 mg·g-1。结论:赤芍药材中芍药苷的含量显著高于亳白芍药材中芍药苷的含量。Objective:A simple,fast,sensitive and accurate HPLC-DAD method was developed for determination of Paeoniflorin in Radix Paeoniae Alba and Radix Paeoniae Rubra.Methods:Chromatographic separations were performed on ZORBAX SB-C18(250 mm × 4.6 mm I.D.,5 μm) column with the isocratic elution of acetonitrile – 0.10% phosphoric acid solution and detection at 230 nm.The column temperature was maintained at 25 ℃,1.0 ml·min-1,R > 1.5,N > 4000.Conclusion:The contents of Radix Paeoniae Alba and Radix Paeoniae Rubra were 25.62、33.46 mg·g-1,respectively.Results: The average content of Paeoniflorin in the Radix Paeoniae Alba was higher than that of in the Radix Paeoniae Rubra significantly.

关 键 词：亳白芍 赤芍 芍药苷 

分 类 号：R28[医药卫生—中药学] R92[医药卫生—中医学]