对映-贝壳杉烷型二萜化合物Leukamenin E对拟南芥愈伤组织磷脂酶D及抗氧化物酶活性的影响  

Effect of Ent-Kaurene diterpenoid Leukameninon E on phospholipase D and antioxidant enzymes from Arabidopsis thaliana callus

作　　者：杨宁[1] 丁芳霞[1] 陈锡莲[1] 王程亮[1] 王丽佩[1] 丁兰[1] 安黎哲[2] 

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]兰州大学生命科学学院,兰州730000

出　　处：《兰州大学学报（自然科学版）》2013年第6期822-827,共6页Journal of Lanzhou University(Natural Sciences)

基　　金：国家自然科学基金项目(31160087;31360061);甘肃省财政厅高校基本科研业务费项目;甘肃省教育厅基金项目(1101-06);西北师范大学学生学术科研资助金项目

摘　　要：以拟南芥愈伤组织为材料,研究磷脂酶D和抗氧化酶系对具有化感潜能的二萜化合物Leukamenin E的响应机制.结果表明:100μmol/LLeukamenin E处理36 h时线粒体膜结合态PLD活性最高,200μmol/L Leukamenin E处理24 h时微粒体膜结合态PLD活性最高.PLD基因实时荧光定量PCR分析表明该基因的表达受Leukamenin E诱导.随着Leukamenin E作用时间的延长,H2O2浓度逐渐升高,SOD,POD,CAT,APX活性总体呈先升高后降低的趋势.说明Leukamenin E能够影响与膜稳定性密切相关的PLD和抗氧化酶活性,这二者都积极响应了Leukamenin E的胁迫.Taking Arabidopsis thaliana callus as material, we studied the mechanism of PLD and its antioxidant enzyme response to the allelochemical potential diterpene compound Leukameninon E. The results showed that these membrane-associated PLD activities in mitochondrial and microsomal peaked at hour 36 and 24 at concentration of 100 μmol/L and 200 μmol/L, respectively. Also, the real-time fluorescent quantitative PCR analyses suggested that PLD gene expression was induced by Leukamenin E. With the prolonging of Leukamenin E treatment, the H2O2 content was raised gradually, the activities of SOD, POD, CAT and APX first increased and then decreased. These indicated that Leukamenin E could influence PLD and antioxidant enzyme activity, which are closely related to the membrane stability and respond positively to Leukamenin E stress.

关 键 词：化感潜能 拟南芥 磷脂酶D RT-PCR 抗氧化物酶 

分 类 号：Q256[生物学—细胞生物学]