核酸酶催化水解核酸的研究  

Study on Hydrolyzationof Nucleic Acid Catalyzed by Nuclease

作　　者：王端好[1] 周河治[2] 

机构地区：[1]湖北工程学院生命科学技术学院,湖北孝感432000 [2]广西大学生命科学与工程学院,广西南宁530004

出　　处：《食品研究与开发》2013年第24期39-41,共3页Food Research and Development

摘　　要：研究核酸酶催化水解核酸生成核苷酸反应条件及用离子交换层析对核苷酸进行分离。最佳水解条件是:RNA的浓度为0.75%,反应液的pH为5,反应温度为55℃,水解时间为2 h;并把4种核苷酸初步分离开。Conditionoptimization of nucleic acidhydrolyzation to nucleotidecatalyzed by nuclease and nu-cleotideseparation by ion-exchange column chromatography were studied in this article. The optimalhydrolyza-tion conditions were the concentration of RNA 0.75%,pH 5, temperature 55℃and time 2 h. Four nucleotides were preliminarily separated.

关 键 词：核酸酶 核酸 条件优化 层析分离 核苷酸 

分 类 号：Q556[生物学—生物化学] Q524