石斛合剂对糖尿病模型大鼠胰高血糖素样肽-1的影响  

The effects of Dendrobium compound on diabtetes model rats about glucagon-like peptide-1

作　　者：高长花[1] 余文珍[1] 施红[1] 刘宏波[1] 郑远燕[1] 

机构地区：[1]福建中医药大学中西医结合学院,福建福州350108

出　　处：《中医临床研究》2013年第23期5-7,共3页Clinical Journal Of Chinese Medicine

基　　金：国家自然科学基金(81072736);福建省自然基金(2010J01192);]陈可冀发展基金会研究课题(CKJ2008017);2008年福建省高校重点建设项目(2008FG-06)

摘　　要：目的:观察石斛合剂对糖尿病模型大鼠高糖负荷后胰高血糖素样肽-1(GLP-1)影响。方法:选取雌性Wistar大鼠随机分为正常对照组(n=10)和观察组(n=30),观察组予高脂高糖饲料喂养4周后腹腔注射小剂量链脲佐菌素(30mg/kg)造模,成模后随机分为模型组(n=10)、石斛合剂组(n=10)、二甲双胍组(n=10)。灌胃8周后,禁食10h行口服葡萄糖耐量(OGTT)试验,尾尖采血,用ELISA法检测血清GLP-1水平。结果:治疗8周后,与模型组比较,石斛合剂组的血糖降低(P<0.01),血清基础(0h)GLP-1水平明显升高(P<0.01),高糖负荷1h后,各组大鼠血清GLP-1水平均升高,石斛合剂组高于模型组与二甲双胍组(P<0.05),且与正常对照组无显著差异;高糖负荷2h后,石斛合剂组、正常对照组大鼠血清GLP-1水平均回落(组内比较,P<0.01),模型组与二甲双胍组则继续上升,各组间比较无差异;高糖负荷3h后,各组大鼠血清GLP-1水平均降低,石斛合剂组血清GLP-1水平较模型组高。结论:石斛合剂能有效的降低糖尿病模型大鼠血糖,增加血清GLP-1水平,其降糖机制可能是通过GLP-1及其降解产物信号途径介导。Objetctive:To observe the effect of Dendrobium compound on serum glucagon-like peptide 1(GLP-1) level in type 2 diabetic rats after high sugar load. Methods:Select female Wistar rats in addition to the normal control group (n=10), give high sugar and high fat diet plus small dose of chain urea with streptozotocin (30mg/kg) to build mold, then randomly divided into model group (n=10), Dendrobium compound group, metformin group (n=10), respectively given saline, Dendrobium compound, metformin for 8 weeks. Fasted for 10h then take oral glucose tolerance test (OGTT), and serum GLP-1 level at each time point were also tested by ELISA. Result:After 8 weeks of treatment, compared with the model group, the BG level of Dendrobium compound group decreased (P<0.01), the level of GLP-1(0h) increased (P<0.01); after high sugar load (1h), the serum GLP-1 levels all increased, compared with model group and metformin group, the serum GLP-1 level of Dendrobium compound group increased (P<0.05), and no significant difference with the control group. After high sugar load (2h), Dendrobium compound group and normal control group serum GLP-1 levels were fallen back (within-group comparisons, P<0.01), and model group and metformin group continued to rise. After high sugar load (3h), the serum GLP-1 levels of all groups decreased. Conclusion:Dendrobium compound can significantly lower blood glucose, increase serum levels of GLP-1, its hypoglycemic mechanism may be mediated by glucagon-like peptide-1and its degradation product signaling pathway.

关 键 词：石斛合剂 糖尿病 OGTT GLP-1 二甲双胍 

分 类 号：R587.1[医药卫生—内分泌]