一重和三重脑震荡大鼠脑固缩的神经细胞定量变化研究  

A Study on the Quantitation of Brain Microglia Cells of Pure Cerebral Concussion and Multiple Cerebral Concussions in Rats

作　　者：曹珍珍[1] 于建云[2,3] 李娟娟[4] 张桓[2] 朱乔[2] 郭泽云[4] 

机构地区：[1]昆明医科大学基础医学实验教学中心,云南昆明650500 [2]昆明医科大学法医学院,云南昆明650500 [3]昆明医科大学脑损伤研究室,云南昆明650500 [4]昆明医科大学人体解剖学与组织胚胎学系,云南昆明650500

出　　处：《昆明医科大学学报》2013年第7期12-16,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(3056004);云南省自然科学基金资助项目(2005C0026Q;2008CD52)

摘　　要：目的建立一重脑震荡(PCC)和三重脑震荡(MCC)大鼠模型,通过计数固缩的神经细胞数目变化,观察PCC和MCC鼠脑的神经细胞病理学变化规律,以探讨MCC大鼠脑损伤的累加效应.方法用自制金属单摆式闭合性脑损伤打击装置复制PCC和MCC大鼠模型96只,随机分为PCC鼠l d、2 d、4 d、8 d、16 d、24 d组和MCC鼠l d、2 d、4 d、8 d、16 d、24 d组(n=6)共12个实验组,另设一正常对照组(n=6).运用HE组织化学染色、尼氏染色,对中隔断面的前额叶皮质(PFC)、梨状皮质(Pi)r、尾壳核(CPu)、伏隔核(AcbC)、终纹床核(BST);背侧海马断面的无颗粒细胞皮质(RSA)、海马CA1-4区、上、下齿状回、丘脑背外侧核(LD);脑干白质以及被盖区等15个脑区进行固缩神经细胞计数.结果大鼠PCC和MCC后绝大多数脑区的固缩神经细胞数目均呈明显上升改变,且MCC后PFC、Pir、CPu、BST、RSA、LD和上齿状回部位的MIG数目上升变化比PCC鼠更加显著(P<0.05).结论大鼠PCC和MCC后绝大多数脑区的固缩神经细胞数目均呈上升性改变,而且MCC后小胶质细胞的升高变化比PCC更加显著,为MCC具有累积性损伤效应提供了病理学依据.Objective To establish Pure Cerebral Concussion(PCC) and Multiple Cerebral Concussion(MCC) rats model and observe the changes of pycnosis nerve cells by cell count after injury,so as to understand the additive effect mechanisms of MCC.Methods PCC and MCC model were duplicated by using a metallic pendulum-striker concussive device.96 female and male Sprague-Dawley rats were randomly divided into twelve groups,namely,2 d,4 d,8 d,16 d,24 d groups and MCC1d,2d,4d,8d,16d,24d groups,and one control group was used.Each group had eight animals(n =6).Each animal was injured and fixed by 4% paraformaldehyde perfused on time.The freezing sections were staining by Hematoxylin and Eosin(HE) and Nissl for microglias cell count in the following areas,namely,PFC,Pir,Cpu,BST,RSA,CA1-4,upper and under dentate gyrus,LD,white matter of brain stem and prerubral region.Result Firstly,the numbers of pycnosis nerve cells in most areas and most test points of both PCC and MCC increased compared with the control group after-injury.Secondly,the quantity of MIG in MCC indicated a more increase compared with PCC in the following areas,namely,PFC,Pir,CPu,BST,RST,CA3,LD and under dentate gyrus(P <0.05).Conclusion The numbers of pycnosis nerve cells in both PCC and MCC increase after injury and then recover gradually.MCC has the accumulation of damage compared with PCC.

关 键 词：一重脑震荡 三重脑震荡 固缩神经细胞 H染色 

分 类 号：R338[医药卫生—人体生理学]