外源性碱性成纤维细胞生长因子诱导下自体静脉桥接修复面神经缺损  

The Vein Graft Technique of the Facial Nerve Regeneration Combined with the Induce of Basic Fibroblast Growth Factor

作　　者：张敏[1] 许彪[2] 江勋 张立亚[2] 

机构地区：[1]云南省第一人民医院/昆明理工大学附属昆华医院口腔矫形科,云南昆明650032 [2]昆明医科大学附属口腔医院,云南昆明650031 [3]云天口腔诊所,云南昆明650031

出　　处：《昆明医科大学学报》2013年第7期25-29,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81260145);云南省科技厅联合专项基金资助项目(2007C0030R)

摘　　要：目的探讨面神经缺损后,经自体静脉桥接修复,并在外源性碱性成纤维细胞生长因子诱导下,神经再生所受到的影响.方法面神经缺损模型采用大耳白兔加以建立.随机分为实验组和对照组.实验组经人造兔面神经缺损,采用其自体静脉桥接,然后在桥接体内注入碱性成纤维细胞生长因子;对照组经人造兔面神经缺损,采用其自体静脉桥接,然后在桥接体内注入生理盐水.于术后100 d将2组兔处死,并对修复的兔面神经进行电生理检测,进而进行组织学观察,以达到对缺损神经再生情况进行评价的目的.结果神经再生及修复表现在2组实验动物均有存在,知识在程度上显现出不同.电生理检测显示,实验组神经传导的速度更快;而组织学检查则显示,实验组无论是再生神经纤维数量还是质量上,均要优于对照组.结论采用外源性碱性成纤维细胞生长因子诱导下自体静脉桥接修复面神经缺损对缺损面神经的再生、修复具有促进作用.Objective To investigate the effect of the vein graft technique combined with the basic fibroblast growth factor(bFGF) on peripheral nerve regeneration.Metheds:12 adult white rabbits were selected and divided into two groups.The facial nerve on the left side of rabbits was served and bridged with the autogenous standard of vein graft.0.3ml bFGF solution(0.75 μg/mL) was injected into the vein graft conduit in the experimental group,while the same amount of saline solution was used in the control group.Microscopic examination and detection of nerve conduct velocity were performed at 100 th day after the operation.Results At 100th day nerve regeneration in the vein graft combined with bFGF showed there were superior functional results of faster conduction velocities(P< 0.05) and distinct histological results compared with the control group.Conclusion The vein graft technique combined with bFGF can induce the facial nerve to regenerate.

关 键 词：碱性成纤维细胞生长因子 静脉桥接 神经缺损 神经再生 

分 类 号：R651.3[医药卫生—外科学]