肾组织肥大细胞表型特点分析研究  

Subtyping of renal mast cells in patients with renal diseases

作　　者：郑敬民[1] 尹广[1] 姚根宏[1] 王建平[1] 刘志红[1] 

机构地区：[1]南京军区南京总医院解放军肾脏病研究所,全军肾脏病重点实验室,江苏南京210002

出　　处：《东南国防医药》2013年第2期97-10,共4页Military Medical Journal of Southeast China

基　　金：江苏省自然科学基金资助项目(BK2012779)

摘　　要：目的阐明各种肾病患者肾组织肥大细胞的表型特点。方法选取包括糖尿病肾病、IgA肾病、急性间质性肾炎、慢性间质性肾炎、过敏性紫癜性肾炎、膜性肾病和狼疮性肾炎在内的肾病患者369例,正常供肾16例,以免疫组化方法检测肾活检标本中肥大细胞类胰蛋白酶和糜蛋白酶表达情况,对其进行分型分析。结果双连续切片分析发现,绝大多数肥大细胞表现为类胰蛋白酶和糜蛋白酶染色双阳性,仅有少数表现为类胰蛋白酶或糜蛋白酶单阳性;"双夹心"连续切片分析法显示,所谓的类胰蛋白酶或糜蛋白酶单阳性肥大细胞实际上是类胰蛋白酶和糜蛋白酶双阳性;以类胰蛋白酶为标记分子和以糜蛋白酶为标记分子检测肾组织肥大细胞的结果比较没有显著区别。结论类胰蛋白酶糜蛋白酶亚型(MCTC亚型)是正常个体和肾病患者肾组织肥大细胞的基本亚型。Objective To determine the subtype of renal mast cells in patients with renal diseases.Methods 369 patients with different renal diseases,including diabetic nephropathy,IgA nephropathy,acute interstitial nephritis,chronic interstitial nephritis,anaphylactoidpurpura nephritis,membranous nephropathy and lupus nephritis,were recruited and 16 normal kidney donors were used as normal controls.Immunohistochemical staining for tryptase and chymase was carried out on the serial renal sections of patients and controls.Mast cells were subtyped based on the expression of tryptase and chymase.Results Analysis based on two serial renal sections showed that most of mast cells were positive for both tryptase and chymase,only a small proportion was single positive.Analysis based on a kind of sandwich serial renal sections demonstrated that the previously found tryptase or chymase single positive mast cells were in fact positive for both tryptase and chymase.Mast cell counting based on immunohistochemical staining for tryptase or for chymase obtained similar results.Conclusion MCTC is the major subtype of renal mast cells.

关 键 词：肥大细胞 肾脏病 肥大细胞亚型 

分 类 号：R329.461[医药卫生—人体解剖和组织胚胎学]