免疫压力下猪瘟病毒遗传稳定性研究  

GENETIC STABILITY OF CLASSICAL SWINE FEVER VIRUS IN THE PRESENCE OF HIGH TITERS OF ANTIBODIES

作　　者：朱妍[1,2] 史子学[3] 涂长春[2] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]军事医学科学院军事兽医研究所,长春130000 [3]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2013年第4期-3-1,共5页Chinese Journal of Animal Infectious Diseases

基　　金：东北农业大学博士启动基金(2010RCB39)

摘　　要：本研究利用抗猪瘟病毒高免血清和猪瘟病毒特异的单克隆抗体充当免疫压力,在免疫压力存在下进行猪瘟病毒的细胞传代,对猪瘟病毒在细胞水平传代过程中是否会发生遗传变异进行研究。结果表明:在免疫压力下,猪瘟病毒在细胞水平进行传代(7代)后,未表现出逃逸抗体中和作用的表型,仍能够被高免血清中和,对传代后病毒的Erns、E2、NS5B基因进行扩增及序列分析后未发现氨基酸的变异。本研究为我国现行的猪瘟防疫政策的执行提供了科学数据,为进一步开展猪瘟病毒遗传变异、病毒演化机制研究奠定了基础。Genetic stability of Classical swine fever virus (CSFV) was studied when the immune pressure was present. The CSFV was grew and passaged 7 times in cell cultures while hyperimmune serum or monoclonal antibodies were added to the growth media. Then the passaged viruses were examined in neutralization and sequencing. Results showed that the passaged viruses were neutralized with no titer changes, indicating stability in cell cultures under immune pressure. Analysis of the full sequences of Erns, E2 and NS5B genes showed no changes of amino acids. Therefore, the present study provided a useful information for evaluating the current immunization policy and understanding on genetic variation and evolution of CSFV.

关 键 词：猪瘟病毒 免疫压力 遗传变异 

分 类 号：S852.651[农业科学—基础兽医学]