普城沙雷氏菌rpoS基因的生物信息学分析  

Bioinformatics analysis of rpoS gene in Serratia plymuthica

作　　者：郭松[1] 刘晓光[1] 

机构地区：[1]江苏大学生命科学研究院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2013年第6期485-488,492,共5页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(NSFC 31240046);江苏省六大人才高峰计划项目

摘　　要：目的:通过生物信息学方法对普城沙雷氏菌G3菌株rpoS基因的序列、理化性质和结构进行解析,为进一步研究生物学功能提供依据。方法:利用Expasy数据库分析蛋白质的理化性质;TMHMMServerv.2.0、TargetP 1.1Server和SignalP 4.1 Server预测跨膜结构和信号肽;分别用NetNGlyc 1.0Server和NetPhos 2.0预测磷酸化、糖基化位点;用SOPMA和SWISS-MODEL分别对蛋白质二级和三级结构进行预测;用MEGA 4 NJ法构建系统进化树。结果:RpoS是一个亲水性蛋白,没有跨膜区域和信号肽,有糖基化位点和高水平的磷酸化位点,α-螺旋和无规则卷曲是其主要二级结构。结论:生物信息学预测表明RpoS具有较高比例的磷酸化位点和由α-螺旋和无规则卷曲组成的二级结构,这与其整合和响应环境中不同的胁迫信号刺激,诱导一般胁迫反应使细菌在逆境中得以生存的生物学功能相吻合。Objective:To analyze the rpoS gene in Serratia plymuthica G3 using bioinformatics approaches to unravel its nucleotide and amino acid sequences,physics,chemistry and structure features with hope in providing help in further studies of the biological function of RpoS in G3.Methods:A series of bioinformatics software were used to predict information on sequence,physical,biochemistry and structure features of RpoS in G3.Results:RpoS was a hydrophilic protein without trans-membrane domain or N-signal peptide.Further analysis revealed that RpoS contained two N-glycosylation sites and 22 phosphrylation sites with a primary secondary structure of α-helix and random coil.Conclusion:The sigma subunit RpoS in strain G3 characterized by a high level of phosphrylation and the secondary structure composed of α-helix and random coil was consistent in its biological function as a signal integrator and network master regulator to be involved in the general stress response in bacteria.

关 键 词：普城沙雷氏菌 RPOS 一般胁迫反应 主控基因 生物信息学 

分 类 号：R378[医药卫生—病原生物学]