胡黄连苷Ⅱ对大鼠脑缺血损伤AQP4、MMP9和COX2表达的影响  

Influence of PicrosideⅡ on the expression of AQP4,MMP9 and COX2 following cerebral ischemic injury in rats

作　　者：敬乃鲁 李晓丹[2] 赵丽[2] 陈红兵[3] 

机构地区：[1]山东省潍坊医学院神经病学教研室,潍坊261000 [2]山东省青岛大学医学院中西医结合中心,青岛266021 [3]山东省潍坊医学院附属益都中心医院,潍坊262500

出　　处：《神经病学与神经康复学杂志》2013年第3期158-161,共4页Journal of Neurology and Neurorehabilitation

基　　金：国家自然科学基金项目(编号:81041092和81274116)

摘　　要：目的：通过正交试验优化胡黄连苷Ⅱ干预大鼠脑缺血模型的治疗剂量和时间窗。方法应用双侧颈总动脉结扎法建立大鼠前脑缺血模型，按照正交试验设计分组，经腹腔注射胡黄连苷Ⅱ干预治疗，酶联免疫吸附法法检测脑组织水通道蛋白4（AQP4）、基质金属蛋白酶9（MMP9）和环氧合酶2（COX2）的含量。结果胡黄连苷Ⅱ干预大鼠脑缺血模型的最佳治疗时间窗和剂量分别为缺血后1．5 h腹腔注射20 mg/kg对于AQP4、缺血后2．0 h和20 mg/kg对于MMP9，缺血后1．5 h和20 mg/kg对于COX2。结论从用药剂量最小化和治疗时间窗最大化的原则综合评价，胡黄连苷Ⅱ干预大鼠脑缺血模型的最佳治疗时间窗和剂量为缺血后1．5～2．0 h腹腔注射20 mg/kg。Objective To optimize the therapeutic dose and time window of Picroside Ⅱ by orthogonal tests in rat cerebral ischemia model . Methods The forebrain ischemia models were established by bilateral common carotid artery occlusion method .The successful models were grouped according to orthogonal experimental design and intervented by injecting Picroside Ⅱ intraperitonenally at different post-ischemia times with different dosages .The contents of aquaporins 4 ( AQP4 ) , matrix metalloproteinases 9 ( MMP9 ) and cyclooxygenase 2 ( COX2 ) in brain tissue were measured by enzyme linked immunosorbent assay . Results The maximal therapeutic time and doses of Picroside Ⅱin rat cerebral ischemia models were 1.5 h post-ischemia with 20 mg/kg for AQP4, 2.0 h post-ischemia with 20 mg/kg for MMP9, and 1.5 h post-ischemia with 20 mg/kg for COX2. Conclusion According to the principle of lowest therapeutic dose with maximum time window , for decreasing the levels of AQP4, MMP9, and COX2 by Picroside Ⅱinjection in the rat ischemia models , optimized therapeutic dose and time window is 20 mg/kg and 1.5~2.0 h post-ischemia.

关 键 词：胡黄连苷Ⅱ 治疗剂量 时间窗 脑缺血 大鼠 

分 类 号：R285.5[医药卫生—中药学]