乌司他丁对外源性过氧化氢介导内皮细胞损伤的保护作用  

The protective effects of ulinastatin against exogenous H_2O_2-induced vascular endothelial cells injury

作　　者：雷兰萍 魏毅君[2] 熊红燕[3] 杨阳 陈涛 金振晓 

机构地区：[1]第四军医大学第一附属医院心血管外科,西安710032 [2]解放军第三医院神经外科,宝鸡721004 [3]西安市中心医院胸心外科,西安710003

出　　处：《中国体外循环杂志》2013年第4期238-241,共4页Chinese Journal of Extracorporeal Circulation

基　　金：陕西省攻关计划项目(2012SF2-21-1;2012K15-02-01);天普研究基金项目(01201104)

摘　　要：目的研究乌司他丁对外源性过氧化氢介导的内皮细胞损伤的保护作用。方法体外培养的人脐静脉内皮细胞(HUVECs)培养液中加入外源性过氧化氢(250μmol/L)制作内皮细胞损伤模型,加入不同浓度的乌司他丁(100、500、1500、3000和5000 U/ml),MTT法检测内皮细胞存活率,同时检测培养液中乳酸脱氢酶(LDH)活性、一氧化氮含量和弹性蛋白酶活性,并测定内皮细胞黏附能力。结果外源性过氧化氢可以显著降低内皮细胞存活率,同时培养液中LDH活性显著升高、一氧化氮含量显著降低、弹性蛋白酶活性显著升高,内皮细胞黏附能力下降。乌司他丁虽然可以在一定程度上抑制细胞培养液中弹性蛋白酶活性的升高,但是对细胞存活率、LDH的释放、一氧化氮的释放、细胞黏附能力均无明显改善作用。结论本研究采用的乌司他丁剂量对外源性过氧化氢介导的内皮细胞损伤无明显保护效果。Objective To investigate the protective effect of ulinastatin on exogenous H2O2-induced vascular endothelial cells injury.Methods In vitro H2O2 mediated endothelial cell injury model was established by adding H2O2(250 μmol/L) into the Human umbilical vein endothelial cells(HUVECs) culture system.Serial concentrations(100,500,1 500,3 000 and 5 000 U/ml) of ulinastatin were added to the culture system.Cellular viability was determined with MTT assay;lactate dehydrogenase(LDH) content,NO content and elastase activity in the culture medium were examined.Cellular adhesive ability was also measured.Results The co-culture process of H2O2 and HUVECs significantly decreased the viability and adhesive ability of HUVECs,decreased the NO content and increased the LDH and elastase content in the culture medium.Ulinatatin inhibited the changes of medium elastase contents at some level,but had no significant effects on cellular viability and adhesive ability.It had no significant effects on the medium content of LDH,NO either.Conclusion Ulinastatin,in the dose used in this study,has no significant protective effects on H2O2 mediated endothelial cell injury.

关 键 词：乌司他丁 内皮细胞 过氧化氢 弹性蛋白酶 

分 类 号：R965[医药卫生—药理学]