非去极化心脏停搏液短时常温保存对猪冠状动脉内皮依赖性舒张功能的影响  

The protective effect of non-depolarizing solution on the endothelium-related relaxation of porcine coronary arteries under normal temperature

作　　者：陈蒙蒙[1] 黑飞龙[1] 吴蓓[1] 王世磊[1] 秦春妮[1] 杨胜男[1] 王晨[1] 龙村[1] 

机构地区：[1]北京协和医学院,中国医学科学院,国家心血管病中心,阜外心血管病医院,心血管疾病国家重点实验室,体外循环科,北京100037

出　　处：《中国体外循环杂志》2013年第4期252-256,共5页Chinese Journal of Extracorporeal Circulation

基　　金：国家自然科学基金资助项目(30972866);北京市自然科学基金资助项目(7102131)

摘　　要：目的探讨短时常温(1h,37℃)条件下,非去极化心脏停搏液(NDP)对猪冠脉内皮源性超极化因子(EDHF)介导的血管舒张功能的影响。方法根据保存液的不同,将血管环分成四组,分别为对照(CON)组、St.Thomas(ST)组、HTK组、NDP组。采用器官槽法,将血管环分别置于Krebs-Hensleit(KH)液、ST液、HTK液、NDP液中,37℃条件下保存1 h,加入一氧化氮合酶阻滞剂N-硝基-L-精氨酸(L-NNA)和环加氧酶阻滞剂(Indomethacin),记录由前列腺素F2α(U46619)及缓激肽(Bradykinin)引起的血管收缩和舒张反应。结果 NDP组中EDHF介导的内皮依赖性血管舒张百分比最大,为(74.79±6.40)%,其余依次为HTK组(51.32±21.92)%、CON组(24.03±8.01)%、ST组(16.20±6.39)%。结论短时常温(1 h,37℃)条件下,NDP液对冠脉内皮的舒张功能具有保护作用,且优于HTK组、CON组和ST组。Objective The present study was designed to evaluate the protective effect of non-depolarizing solution(NDP) on the endothelium-derived hyperpolarizing factor(EDHF)-mediated relaxation of porcine coronary arteries after rings were incubated with NDP solution at 37℃ for 1 hour.Methods Due to different preservation solutions used in different groups,the rings were divided into four groups:control(CON) group,St.Thomas(ST) group,Histidine-Tryptophan-Ketoglutarate(HTK) group and NDP group.Rings from porcine coronary arteries were studied in the organ chamber.After equilibration,the rings were incubated with Krebs-Helenseit solution in CON group,St.Thomas solution in ST group,HTK solution in HTK group,non-depolarizing solution in NDP group at 37℃ for 1hour.Then Pre-contraction tone induced by prostaglandin F2α(U46619) and endothelium-dependent relaxation tone induced by bradykinin was measured in the presence of indomethacin and L-NNA.Results After incubation with different solutions at 37℃ for 1hour,the EDHF-mediated relaxation induced by bradykinin was much higher in the NDP group 74.79%±6.40% than in the HTK group 51.32%±21.92%,CON group 24.03%±8.01% and ST group 16.20%±6.39%.Conclusion NDP solution provides superior endothelium protective effect compared with ST and HTK solution under normal temperature.

关 键 词：血管环 保存液 内皮源性超极化因子 

分 类 号：R654.1[医药卫生—外科学]