浙江省首例人感染H7N9禽流感病例的实验室检测与分析  

Detection and molecular characterization of novel avian-origin influenza A (H7N9) virus isolated from the first human case in Zhejiang

作　　者：孙永祥[1] 陈棋炯[1] 李钧[2] 于新芬[2] 潘劲草[2] 

机构地区：[1]311201,杭州市萧山区疾病预防控制中心卫生实验中心 [2]310021,杭州市疾病预防控制中心微生物检验科

出　　处：《国际流行病学传染病学杂志》2013年第3期-,共4页International Journal of Epidemiology and Infectious Disease

摘　　要：目的 通过对杭州市萧山区1例疑似人感染禽流感病人咽拭子和鼻腔冲洗液样本进行检测和分析,加深对A型流感病毒抗原变异特性的认识,进一步提高实验室相应的检测能力.方法 以实时荧光定量PCR(real-time PCR)检测流感病毒核酸,对未能分型的HA、NA和M基因通过逆转录PCR(RT-PCR)扩增而后克隆测定;同时接种狗肾细胞(MDCK)进行病毒培养.结果 样本A型流感病毒核酸检测为阳性,但未能分型;HA、NA、M基因测序结果表明与禽源H7、N9、M基因相似性最高;同时A型流感病毒培养也呈阳性.结论 经过浙江省CDC与中国CDC证实,该患者感染H7N9禽流感病毒,为浙江省首个病例.Objective To detect and analyze the throat swab and nasal cavity lavaging fluid samples from one suspected human avian influenza in Xiaoshan,in order to deepen the understanding of the antigenic variation characteristics of influenza A virus,and to strengthen laboratory diagnostic ability.Methods The influenza virus nucleic acid from the samples were detected by real-time fluorescent quantitative PCR (real-time PCR).In addition,unallocated HA,NA and M genes were amplified by reverse transcription PCR (RT-PCR) and sequenced after cloning.Meanwhile,MDCK cells were inoculated with the sample for viral culturing.Results RNA extracted from the sample was positive for influenza A virus nucleic acid,but could not be typed.Furthermore,the results of gene sequencing revealed that the HA,NA,M genes were most closely related to H7,N9,M genes of avian origin,respectively,and the viral culturing was also positive for influenza A virus.Conclusions The patient was infected with a novel avian-origin influenza A (H7N9) virus,and was the first case in Zhejiang Province.

关 键 词：禽流感 H7N9 实时荧光PCR法 序列分析 

分 类 号：R51[医药卫生—内科学]