新型脊髓纳米组织工程支架的组织相容性  

Histocompatibility of new nano-scaffolds for spinal tissue engineering

作　　者：周继辉[1,2] 姚猛[3] 王岩松[3] 隋福革[1,2] 刘玉刚[3] 赵丛然[1,2] 田飞鹏[1,2] 

机构地区：[1]大庆龙南医院 [2]齐齐哈尔医学院附属第五医院,黑龙江省大庆市1634532 [3]哈尔滨医科大学附属第二医院脊柱外科,黑龙江省哈尔滨市150086

出　　处：《中国组织工程研究》2013年第21期3854-3861,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(30800260);黑龙江省自然科学基金项目(D200916;D200902)~~

摘　　要：背景:纳米技术可改善脊髓组织工程生物材料的性能。目的:分析新型脊髓纳米组织工程支架的组织相容性。方法:以胶原为原料制备纤维定向排列及非定向排列的纳米纤维膜,培养及鉴定SD大鼠脊髓源性神经干细胞。将两种纳米纤维膜与SD乳鼠脊髓源性神经干细胞共培养,以正常培养的神经干细胞为对照,通过MTT实验检测纳米纤维膜的细胞相容性;以扫描电镜检测细胞在纳米纤维膜表面的黏附及增殖情况;将纳米纤维膜植入SD大鼠体内,通过组织学检查确定其降解情况及组织相容性;通过免疫组织化学实验确定神经干细胞在体内的存活及移动情况。结果与结论:两种纳米纤维膜表面的神经干细胞黏附及增殖情况良好,MTT实验结果表明纳米纤维膜的细胞相容性佳,电镜结果表明细胞在纳米纤维膜表面黏附良好,增殖情况佳;在体内纳米纤维膜降解情况良好,组织相容性佳;BrdU标定的神经干细胞在SD大鼠体内存活并移动情况良好。结果表明新型纳米组织工程支架具有良好的细胞及组织相容性。BACKGROUND: The properties of tissue engineering materials have been improved obviously with the rise and development of nanotechnology, the prospect of which is broad in spinal cord tissue engineering. OBJECTIVE: To observe the histocompatibility of new nano-scaffolds for spinal tissue engineering. METHODS: Electrospun aligned and randomly oriented nano fibrous scaffolds were made of collagen. Spinal cord derived neural progenitor cells from Sprague-Dawley rats were cultured and identified, and then the cells were cultured on the aligned and randomly oriented col agen nanofibrous scaffolds. Neural stem cells under normal culture served as controls. Scanning electron microscope was used to observe the cells’ adhesion and generation on the scaffold surface, and cel histocompatibility was determined with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Histocompatibility and degradation of nanofibrous scaffolds in vivo were determined through histological observation. The survival and movement of neural stem cells in the body were determined by immunohistochemistry experiments. RESULTS AND CONCLUSION: Superficial morphous of electrospun aligned and randomly oriented col agen nanofibrous scaffolds were in accordence with contrivable requisition. The cel adhesion and proliferation on the surface of the col agen nanofibrous scaffolds was perfect. Results from 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay showed that the cell ular compatibility of nano-scaffolds was good. Nanofibrous scaffolds degraded wel and their histocompatibility in vivo was good. The 5-bromo-2''-deoxyuridine-labeled cells in vivo survived and migrated wel . Histocompatibility of new nano-scaffolds for tissue engineering is satisfactory.

关 键 词：生物材料 纳米生物材料 脊髓 组织工程 纳米支架 纳米纤维膜 组织相容性 神经干细胞 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]