骨形态发生蛋白2/注射式硫酸钙缓释载体的生物相容性  

Biocompatibility of bone morphogenetic protein-2/injectable calcium sulfate cement slow-release carriers

作　　者：陈琛[1] 徐晓峰[2] 

机构地区：[1]铜陵市人民医院,安徽省铜陵市244000 [2]江苏大学附属医院骨科,江苏省镇江市212001

出　　处：《中国组织工程研究》2013年第21期3921-3927,共7页Chinese Journal of Tissue Engineering Research

基　　金：镇江市社会发展基金资助项目(SH2002019)课题名称:骨髓间质干细胞体外扩增回植对骨缺损的作用~~

摘　　要：背景:目前硫酸钙主要被作为抗生素载体和成骨因子载体。目的:观察骨形态发生蛋白2/注射式硫酸钙缓释系统的体外缓释效果及与种子细胞的生物相容性。方法:制备骨形态发生蛋白2/注射式硫酸钙缓释系统,检测其体外释放性能。将SD大鼠骨髓间充质干细胞经体外诱导培养、扩增后,种植于骨形态发生蛋白2/注射式硫酸钙缓释载体和单纯的注射式硫酸钙支架上行体外培养。结果与结论:骨形态发生蛋白2/注射式硫酸钙缓释支架具有缓释骨形态发生蛋白2的效果,持续时间可达31d。骨形态发生蛋白2/注射式硫酸钙缓释支架与大鼠骨髓间充质干细胞具有优良的生物相容性,并且相同时间点支架上的细胞黏附率、细胞数量及细胞碱性磷酸酶活性均明显高于注射式硫酸钙支架;扫描电镜发现两组材料上均有细胞生长,骨形态发生蛋白2/注射式硫酸钙缓释支架上的细胞在支架表面和孔隙内生长良好,细胞突起接触融合,细胞密集区域可见细胞外基质形成,大量细胞包绕在材料表面;注射式硫酸钙支架上的细胞黏附数量较少,生长情况不及骨形态发生蛋白2/注射式硫酸钙缓释支架上的细胞。BACKGROUND: The calcium sulfate is mostly used as carriers for antibiotics and osteogenic factors by now. OBJECTIVE: To observe the in vitro delivery effect of bone morphogenetic protein-2/injectable calcium sulfate cement slow-release system and the in vitro biocompatibility with bone marrow mesenchymal stem cel s. METHODS: Bone morphogenetic protein-2/injectable calcium sulfate cement slow-release scaffold was prepared and its release properties were detected. Bone marrow mesenchymal stem cel s obtained from Sprague-Dawley rats were induced, cultured and proliferated in vitro, and then, the cel s were seeded onto the bone morphogenetic protein-2/injectable calcium sulfate cement scaffolds (experimental group) or single injectable calcium sulfate cement scaffolds (control group). RESULTS AND CONCLUSION: The release of bone morphogenetic protein-2 on the scaf olds of experimental group could last for 31 days. Rat bone marrow mesenchymal stem cel s had a good biocompatibility with the bone morphogenetic protein-2/injectable calcium sulfate cement scaf olds. The adhesive rate, cel number and alkaline phosphatase activity at the same time point in the scaf olds of experimental group were significantly greater than those of the control group. Cel s could be observed on each scaf old under a scanning electron microscope. In the experimental group, interconnected cel processes were fused, extracel ular matrix was visible in the dense area, and the scaf old was surrounded by a large number of cel s. The cel s in experimental group grew bet er than the control group.

关 键 词：生物材料 材料生物相容性 骨形态发生蛋白2 注射式硫酸钙 大鼠骨髓间充质干细胞 生物相容性 其他基金 

分 类 号：R318[医药卫生—生物医学工程]