磁性复合骨水泥的体外细胞毒性  

Cytotoxicity of the magnetic bone cement in vitro

作　　者：唐正海[1] 王晓文[2] 张友仁[1] 潘琳[3] 唐劲天[2] 岳秉飞 

机构地区：[1]北京中医药大学,北京市100102 [2]清华大学玉泉医院脑神经疾病研究所,北京市100049 [3]中日友好医院临床医学研究所,北京市100029 [4]中国药品生物制品检定所,北京市100050

出　　处：《中国组织工程研究》2013年第21期3937-3943,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81071885)~~

摘　　要：背景:磁性复合骨水泥作为一种新型骨转移治疗材料,不仅能对骨缺损进行修复,还能在交变磁场下升温杀死骨肿瘤。目的:检测磁性复合骨水泥对小鼠L929成纤维细胞增殖活性的影响及细胞毒性反应。方法:采用CCK-8法检测含0%(即磷酸钙骨水泥组),10%,20%Fe3O4的磁性磷酸钙骨水泥浸提液及含0%(即聚甲基丙烯酸甲酯骨水泥组),10%,20%Fe3O4的聚甲基丙烯酸甲酯骨水泥浸提液对对数生长期小鼠L929成纤维细胞的毒性作用,每种浸提液又分为50%与100%两个浓度梯度;以正常培养基培养的小鼠L929成纤维细胞作为阴性对照组。结果与结论:①在100%浓度梯度下:除含20%Fe3O4的磁性聚甲基丙烯酸甲酯骨水泥组细胞数量较少、细胞形态异常外,其余磁性聚甲基丙烯酸甲酯骨水泥组细胞生长状态良好;含10%Fe3O4的磁性磷酸钙骨水泥组培养24h时表现出轻度细胞毒性,毒性2级;含20%Fe3O4的聚甲基丙烯酸甲酯骨水泥在24-72h为表现出轻、中度细胞毒性,毒性为2,3级。各磁性磷酸钙骨水泥组细胞数量及形态正常。②在50%浓度梯度下:各组细胞形态均正常,细胞毒性为0级或1级。表明磁性聚甲基丙烯酸甲酯骨水泥具有轻、中度细胞毒性,磁性磷酸钙骨水泥细胞相容性良好,基本无细胞毒性。@@@@BACKGROUND: Magnetic bone cement has been used as a new kind of material for bone metastasis, and it can repair bone defects and cure bone tumors with temperature rising under an alternating magnetic field. OBJECTIVE: To test the magnetic bone cement effects on cytotoxicity and cell proliferation of mouse L929 cells. METHODS: The cytotoxicity of magnetic calcium phosphate cement and polymethylmethacrylate bone cement leaching liquors, respectively, containing 0, 10%, 20% Fe3O4, to L929 cells at logarithmic growth phase was detected using Cell Count Kit-8 assay. Two kinds of leaching liquors were prepared to two concentration gradients, 50% and 100%. Mouse L929 cells cultured in normal culture medium were considered as control group. RESULTS AND CONCLUSION: (1) Under 100% concentration gradient: Cell s grew wel in polymethyl methacrylate bone cement groups containing 0, 10% Fe3O4 except for 20% Fe3O4. Magnetic calcium phosphate cement containing 10% Fe3O4 showed mild cell toxicity, graded as 2, after culture for 24 hours. During 24- 72 hours, polymethylmethacrylate bone cement containing 20% Fe3O4 exhibited mild or moderate cell toxicity and the toxicity was graded as 2 or 3. (2) Under 50 concentration gradient: Cells grew wel in al groups. The cell toxicity was graded as 0 or 1. Magnetic polymethylmethacrylate bone cement has mild or moderate cell toxicity and magnetic calcium phosphate cement exhibits a good biocompatibility with no cell toxicity.

关 键 词：生物材料 组织工程骨材料 磁性骨水泥 聚甲基丙烯酸甲酯 磷酸钙 生物相容性 成纤维细胞 细胞毒性 增殖 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]