肝素抗凝材料联合血管束植入修复骨缺损的血管化  

Vascularization of heparin-chitosan-coated acellular bone matrix combined with vascular bundles in repair of segmental bone defects

作　　者：张春梅[1] 田洪玲 赵洁[1] 腾云[1] 余绍芬 孙新君[1] 

机构地区：[1]解放军第八十九医院创伤骨科研究所,山东省潍坊市261021 [2]济南军区联勤部物资采购站卫生所,山东省济南市250022

出　　处：《中国组织工程研究》2013年第21期3944-3951,共8页Chinese Journal of Tissue Engineering Research

基　　金：全军十一五课题(06MA096)~~

摘　　要：背景:前期研究显示肝素-壳聚糖脱细胞骨基质材料植入骨缺损受区后,材料内部血液供应明显改善,将血管束植入大体积抗凝材料能否促进骨缺损处血液灌注和血管化进程?目的:观察局部抗凝的肝素-壳聚糖脱细胞骨基质材料联合血管束植入对骨缺损修复早期血液灌注及血管化的影响。方法:取25只健康新西兰大白兔制作双侧桡骨中段20mm长骨缺损模型,右侧植入局部抗凝的肝素-壳聚糖脱细胞骨基质材料联合自体血管束(实验组),左侧植入脱细胞骨基质材料联合自体血管束(对照组),术后1,3,7,14,28d行CT灌注成像及组织学观察。结果与结论:术后1d开始,实验组血容量、血流量显著高于对照组(P<0.05),且实验组材料中心部位有明显血液灌注,对照组植入物周围有血液渗透,此种趋势持续到术后28d。术后1d,实验组复合材料中有大量红细胞和有核细胞,对照组以渗透液体为主,偶见细胞;术后3-7d,实验组复合材料网孔中有血管形成,之后逐渐增多,对照组见植入血管血栓形成、管腔闭塞,周围组织纤维化包裹,实验组各时间点材料内部血管数高于对照组(P<0.05)。提示局部抗凝的肝素-壳聚糖脱细胞骨基质材料可促进血管束植入后材料内部的血液灌注和早期血管化进程。BACKGROUND: Preliminary studies have shown that heparin-chitosan-coated acel ular bone matrix implantation can significantly improve the blood supply of bone defect region. Then, large-volume anticoagulant material implantation into vascular bundles can improve blood perfusion and vascularization processes or not? OBJECTIVE: To investigate the effects of heparin-chotisan-coated acel ular bone matrix implantation combined with vascular bundles on hemoperfusion and vascularization in the early period of bone defects. METHODS: Twenty-five healthy New Zealand white rabbits were selected to prepare 20 mm long bone defect models of the bilateral radi . Heparin-chotisan-coated acel ular bone matrix combined with vascular bundles (experimental group) was implanted into the right radius, and acel ular bone matrix combined with vascular bundles (control group) was implanted into the left radius. CT perfusion imaging and histological observation were done at postoperative days 1, 3, 7, 14, 28. RESULTS AND CONCLUSION: Blood volume and blood flow were significantly higher in the experimental group than the control group from postoperative day 1 til day 28 (P < 0.05). Blood perfusion at the center of implant material and blood infiltration around the implant were found in the experimental and control groups, respectively. A large amount of red blood cel s and nucleated cel s within the composite material were visible in the experimental group, while permeated liquid was mainly seen in the control group at 1 day postoperatively. Within 3-7 days after implantation, new vessels appeared in the micropores of the experimental group and then increased gradual y; in the control group, vascular thrombosis and luminal occlusion formed in the implanted vessels, wrapped with fibrotic surrounding tissues. The number of vessels within the implant was higher in the experimental group than the control group at each time point (P < 0.05). It indicates that implantation of heparin-chitosan-coated acel ular bone matrix scaffold combined with va

关 键 词：生物材料 生物材料基础实验 组织工程骨 预血管化 肝素 壳聚糖 脱细胞骨基质骨缺损 血管束植入 其他基金 

分 类 号：R318[医药卫生—生物医学工程]