粗皮侧耳粗毒素的提取及杀线虫活性的研究  

Nematicitdal Activity and Elementary Characteristics of the Crude from Pleurotus ostreatus

作　　者：向红琼[1] 冯志新[1] 

机构地区：[1]华南农业大学植物线虫研究室,广州510642

出　　处：《云南农业大学学报》1999年第S1期82-87,共6页Journal of Yunnan Agricultural University

摘　　要：粗皮侧耳 (Pleurotusostreatus)菌物能产生杀线虫毒素 .用乙酸乙酯从该菌丝麦粒培养物中提取的粗毒素具有杀线虫活性 ,稀释 10倍 ,击倒 95 %的线虫 (Panagrellusredivivus)仅需4 5h .该菌产生的毒素可溶于水及各种有机溶剂 ,对热具有高度的稳定性 。Pleurotus ostreatus is a commone boasidiomyct in the world.The hyphae of Pleurotus ostreatus can produce nematotoxin. The nematicidal activity compenent was detected in the ethyl acetate crude extract from P osrteatus hyphae cultured on moistened,autoclaved wheat.When thecrude PO-toxin was diluted 10X,95% of nematodes were immobilizied within 4 h and 30 minutes.The nematotoxin of P ostreatus dissolves in water and in acetone,ethyl acetate,methanol,but the nematicidal activity was not detected in the Petroleum ether.It had activity to many nematodes, such as Hirschmanniella oryzae, Bursaphelenchus xylophilus, Pratylenshus sp., Meloidogyne incognita, M.arenaria and so on.

关 键 词：粗皮侧耳 杀线虫粗毒素 粗毒素基本特性 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]