IFNγ/IL-4对人胎盘滋养层功能的影响  

Immune regulation of IFNγ/IL-4 on human trophoblast in vitro

作　　者：王越[1] 阎玉文[2] 王金兰[1] 王卫国[3] 

机构地区：[1]武警医学院免疫学教研室,天津300162 [2]武警医学院寄生虫学教研室,天津300162 [3]武警医学院物理学教研室,天津300162

出　　处：《武警医学院学报》2004年第4期272-275,共4页Acta Academiae Medicinae CPAPF

基　　金：武警医学院科研基金课题 (WY2 0 0 1-14)

摘　　要：【目的】研究γ干扰素 (IFNγ) /白细胞介素 4 (IL 4 )对人孕早期胎盘滋养层细胞活性及人绒毛膜促性腺激素 (hCG)分泌的影响。【方法】胎盘滋养层细胞活性采用MTT法进行检测 ,胎盘滋养层组织分泌的hCG采用放免法进行分析测定。结果 :IFNγ在一定浓度范围内 (10～ 10 0 0ng/ml)对胎盘滋养层细胞活性及其hCG分泌有明显的抑制作用 (P <0 0 1) ;而IL 4 (1～ 10ng/ml)则有明显的促进作用 (P <0 0 1)。 【结论】IFNγ/ILObjectiveTo study effect of IFNγ/IL-4 on viability of human trophoblastic cells and secretion of hCG(human Chorionic Gonadotropin) by trophoblastic tissues in early pregnancy.The viability of trophoblastic cells were detected by MTT.Radioimmunoassay were applied to determine secretion of hCG by trophoblastic tissues.A ranged dose of IFNγ(10～1 000 ng/ml) can significantly inhibit viability of trophoblastic cells and secretion of hCG(P<0.01),IL-4(1～10 ng/ml) can significantly enhance the above trophoblastic functions(P<0.01).[Conclusion]IFNγ/IL-4 play a important role of immune regulation in early pregnancy by influencing viability of trophoblastic cells and secretion of hCG likely.

关 键 词：IFNγ/IL-4 胎盘滋养层 人绒毛膜促性腺激素 免疫调节 

分 类 号：R714.12[医药卫生—妇产科学]