大黄5个蒽醌类成分与大鼠血浆蛋白结合率的测定  

Determination of the Binding Rate of Rat Plasma Protein with Five Anthraquinones in Rhubarb

作　　者：冯素香[1,2] 吴加 李建生[1] 屈凌波[2,3] 徐会平[2] 杨冉[2] 李建军[2] 

机构地区：[1]河南中医学院,郑州450008 [2]郑州大学,郑州450001 [3]河南工业大学,郑州450001

出　　处：《中国现代应用药学》2013年第1期1-5,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金资助项目(81274179);河南省高校青年骨干教师资助项目(2009GGJS-065);河南省教育自然科学研究计划项目(2010A360002)

摘　　要：目的建立测定大黄苷元中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚5种成分与大鼠血浆蛋白结合率的方法。方法采用体外平衡透析法,模拟大黄苷元与血浆蛋白的结合过程,以固相萃取柱处理血浆样品,用高效液相色谱法测定大黄苷元中5种蒽醌类成分在透析袋内血浆中的浓度与透析袋外缓冲液中的浓度,计算血浆蛋白结合率。结果芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚提取回收率分别为68.6%,91.5%,78.6%,88.2%和71.4%,与大鼠体外血浆蛋白结合率分别为93.1%,91.3%,95.2%,87.5%和89.7%。结论大黄5种苷元与大鼠血浆蛋白结合率较高,且蛋白结合率与血药浓度无明显依赖性。OBJECTIVE To develop a method for the simultaneous determination of the binding rate of plasma protein with aloe-emodin,rhein,emodin,chrysophanol and physcion.METHODS The equilibrium dialysis method was carried out to imitate the binding process between five anthraquinones and plasma protein.Plasma samples were treated with solid-phase extraction and the binding rate was determined by HPLC.RESULTS The extract recoveries of aloe-emodin,rhein,emodin,chrysophanol and physcion were 68.6%,91.5%,78.6%,88.2% and 71.4%,the binding rates of plasma protein were 93.1%,91.3%,95.2%,87.5% and 89.7%.CONCLUSION This method shows the high binding rate of five anthraquinones to plasma protein.The binding rates of five anthraquinones are not proportionally dependent on plasma concentration.

关 键 词：大黄苷元 血浆蛋白结合率 平衡透析法 高效液相色谱法 

分 类 号：R285.5[医药卫生—中药学]