高效液相色谱法测定三黄系列品种中盐酸小檗碱的含量  

Determination of Berberine Hydrochloride in Sanhuang series by HPLC

作　　者：刘辉[1] 刘永利 

机构地区：[1]常州市武进中医医院,江苏常州21316 [2]河北省食品药品检验院,河北石家庄050011

出　　处：《中国药物经济学》2013年第S1期26-28,共3页China Journal of Pharmaceutical Economics

基　　金：河北省自然科学基金资助项目:中成药质量标准模式创新研究(项目号:C2011320007)

摘　　要：目的建立三黄片、三黄胶囊等系列品种中盐酸小檗碱的HPLC含量测定方法。方法 C18柱(4.6×150mm,5μm,Capcell pak C18 MGⅡ),以乙腈-水(1:1)(每1000ml流动相中含磷酸二氢钾3.4g,十二烷基硫酸钠1.7g)为流动相,检测波长为265nm,流速为1ml min-1,柱温为室温。结果此方法线性关系良好,平均加样回收率为99.54%。结论本方法分离度好,结果准确可靠。Objective To establish the determination method of Berberine hydrochloride in Sanhuang tablet and Sanhuang capsule by HPLC. Methods The C18 column(4.6×150mm,5μm, Capcellpak C18 MGⅡ)was used in this method,and a mixture acetonitrile-water(1:1)(3.4g pltassium di hydrogen phosphate and 1.7g sodium dodecyl sulphate were in 1000ml mobile phase) as a mobile phase, detection wavelength 265nm. Results This method showed a good linear relationship, the mean recovery was 99.54%. Conclusion This method showed a good separating degree and reliable.

关 键 词：HPLC 盐酸小檗碱 三黄片 三黄胶囊 

分 类 号：R286[医药卫生—中药学]