骨搬移技术治疗骨不连的分子机制体外实验动物研究-新型力学加载式生物反应器对雄性大鼠成骨细胞OPG/RANKL表达的影响  

Move technique in the treatment of bone nonunion vitro animal studies the molecular mechanisms-new mechanical loading bioreactor in male rat osteoblast OPG/RANKL expression

作　　者：于大淼[1] 廉永云[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,150001

出　　处：《环球中医药》2013年第S1期28-29,共2页Global Traditional Chinese Medicine

摘　　要：目的新型力学加载式生物反应器相同振动频率和不同振动应变作用于体外三维支架条件下培养的雄性大鼠成骨细胞,探究OPG/RANKL表达的影响。方法雄性大鼠成骨细胞培养、传代后分组:振动频率为1.0Hz,不同振动应变(A B C三组)及对照组(无振动应变及振动频率)(D组)4组。应用RT-PCR和Western blot检测干预后OPG和RANKL mRNA和蛋白水平的表达。结果仅振动应变2000με(B组)作用于细胞后,细胞中OPGmRNA蛋白水平表达升高,RANKLmRNA及蛋白水平则降低(均P<0.05)。其余实验组未见阳性反应。结论通过新型力学加载式生物反应器(振动应变2000με振动频率1.0Hz)的压应力作用于细胞后可以正性调节OPG/RANKL轴的表达。Objective to model the same vibration frequency of the mechanical loading bioreactor and different vibration strain effect on the three-dimensional scaffold in vitro cultured osteoblast in male rats,influence on OPG/RANKL expression.Methods the male rat osteoblast culture,subculture group:vibration frequency is 1Hz,the different vibration strain(A B C three group)and control group(no vibration strain and vibration frequency)(group D)of 4groups.Expression of OPG and RANKL mRNA and protein levels of RT-PCR and Western blot detection after intervention.Results only vibration strain 2000με(group B)acting on cells,elevated levels of OPGmRNA protein expression in RANKLmRNA cells,and the protein level decreased(all P<0.05).The experimental group had no positive reaction.Conclusion by the new type of mechanical loading bioreactor(vibration strain 2000μεvibration frequency 1Hz)pressure expression for the stress acting on cells can positively regulate the OPG/RANKL axis.

关 键 词：成骨细胞 新型力学加载式生物反应器 OPG/RANKL 

分 类 号：R318.01[医药卫生—生物医学工程]