土壤硝态氮自动检测平台研究  

Automatic Detection Platform for Soil Nitrate-nitrogen

作　　者：张丽楠[1] 张淼[1] 蒲攀[1] 张集恬 

机构地区：[1]中国农业大学现代精细农业系统集成研究教育部重点实验室,北京100083

出　　处：《农业机械学报》2013年第S2期220-225,共6页Transactions of the Chinese Society for Agricultural Machinery

基　　金：国家自然科学基金资助项目(31201136;61134011);高等学校博士学科点专项科研基金资助项目(20110008120023)

摘　　要：为了快速、低成本获取土壤硝态氮含量,以自制硝酸根掺杂聚吡咯(PPy(NO-3))离子选择电极为传感手段,自主开发了多通道土壤硝态氮自动检测平台。以实验室配制的质量浓度为1~250 mg/L的硝态氮标准溶液为实验材料,分别对平台响应时间、清洗次数对平台标定及土壤样本检测结果的影响进行了研究。实验结果显示,平台系统用于土壤硝态氮检测时的响应时间为40 s;进行系统标定和土壤浸提溶液检测时,用下一待测溶液对平台进行清洗2次即可达到理想效果,此应用方式下得到的平台检测结果与手动方式测得的结果相当,且平台的不同检测通道之间具有相似的检测性能和良好的一致性。基于20个土样的系统验证结果显示,平台系统的土壤硝态氮检测结果与紫外分光光度法检测结果之间具有较好的相关性,决定系数为0.91。In order to detect soil nitrate-nitrogrn content rapidly with low cost,an automatic multichannel detection platform for soil nitrate-nitrogrn was developed.The self-designed PPy(NO-3) ion selective electrode(ISE) was used to sense the nitrate ion in solutions.The platform response time,effects of rinsing time on platform calibration and soil nitrate-nitrogen detection were discussed with a series of standard nitrate solutions,nitrate-nitrogen contents of which were ranging from 1~250 mg/L.Testing results showed that response time of the platform was 40 s when it was used in soil nitrate-nitrogrn detection.During system calibration and soil nitrate extracts determination,rinsing operation for the system could be carried out directly with the next sample solution.And rinsing 2 times could achieve a desirable effectiveness.No significant differences were got between the results detected separately by the platform and manual operation.Also,different channels of the platform possessed similar detection performances and good consistency.The system validation results conducted with 20 soils indicated that satisfied correlation was obtained between the soil nitrate-nitrogen results detected with the platform and ultraviolet spectrophotometric method.The determination coefficient(R2) was 0.91.

关 键 词：硝态氮 土壤自动检测平台 离子选择电极 测土配方施肥 

分 类 号：S153.6[农业科学—土壤学]