金鱼微卫星标记的筛选、引物设计及应用  

Microsatellite Marker's Isolation,Primer Design and Application in the Goldfish

作　　者：张升利[1] 杨璞[1] 梁拥军[1] 孙向军[1] 孙砚胜[1] 苏建通[1] 史东杰[1] 

机构地区：[1]北京市水产科学研究所,北京100068

出　　处：《华北农学报》2013年第S1期27-31,共5页Acta Agriculturae Boreali-Sinica

基　　金：北京市自然科学基金项目(6112008);现代农业产业技术体系北京市观赏鱼创新团队建设项目;国家科技基础条件平台建设运行项目(北京地区水产种质资源标准化整理;整合与共享);农业部都市农业(北方)重点实验室项目(99124120)

摘　　要：利用磁珠富集法分离草金鱼的微卫星分子序列,获得38个阳性克隆,对其进行测序,获得31个微卫星DNA序列,利用软件Primer Premier 5.0设计引物12对,其中有8对引物可扩增出稳定的目的条带。利用筛选出的8对微卫星引物对北京2个草金鱼养殖群体进行了遗传多样性分析。结果表明,2个草金鱼养殖群体的遗传多样性水平较高,其平均观测杂合度(Ho)分别为0.565 5,0.534 3,平均期望杂合度(He)分别为0.657 0,0.628 6,平均多态性信息含量PIC分别为0.510 6,0.500 9。两群体间遗传距离为0.095 7,遗传相似度为0.921 1。8个微卫星位点可用于金鱼群体的遗传学研究。Microsatellite DNA was isolated from goldfish with combining biotin capture method .We obtained 38 positive clones and isolated 31 microsatellite sequences .12 pairs of primers were designed by Primer Premier 5 .0 and 8 pairs of primers can obtain amplifying products among them .The genetic diversity of the artificial breed gold-fish populations were studied by using the 8 Microsatellite primers .The results showed that the genetic diversity lev-el of two populations were high.The average heterozygosity(Ho) in two populations was from 0.534 3 to 0.565 5, average excepted heterozygosity (He) was from 0.628 6 to 0.657 0,and average polymorphism information content (PIC) was from 0.500 9 to 0.510 6.The genetic distance was 0.095 7 and genetic identity was 0.921 1.All of 8 microsatellite locus could be used for genetic analysis in goldfish .

关 键 词：金鱼 微卫星 磁珠富集 遗传多样性 

分 类 号：S917.4[农业科学—水产科学]