花生网斑病诱导抗性及寄主防御酶应答反应和NPR1表达的测定  

Peanut Web Blotch Induced Resistance and the Host Defense Enzyme Response and Determination of NPR1 Expression

作　　者：夏淑春[1] 王学武 王志葵 况川[1] 张茹琴[1] 鄢洪海[1] 

机构地区：[1]青岛农业大学农学与植物保护学院,山东青岛266109 [2]青岛市植物保护站,山东青岛266101 [3]青岛市即墨植物保护站,山东青岛266110

出　　处：《华北农学报》2013年第S1期388-392,共5页Acta Agriculturae Boreali-Sinica

基　　金：山东省科技发展项目(2009GG10009022);山东省自然科学基金项目(ZR2011CL005);山东省"泰山学者"建设工程专项经费(BS2009NY040)

摘　　要：采用叶面喷施水杨酸、病原菌培养滤液、草酸等因子诱导花生植株对网斑病抗病性,并测定寄主防御酶活性变化和NPR1基因表达量,来探讨花生诱导抗病性及作用机制。结果表明:诱导处理可以使花生获得系统抗病性,且以2.0 mmol/L水杨酸诱导花生网斑病抗性效果最好,控制病害效果达到35.3%;寄主防御酶活性升高与诱导抗病性强度呈正相关,但几种防御酶在诱导抗病性应答反应中的变化存在显著差异;实时荧光定量PCR分析表明:2.0mmol/L的SA诱导处理后花生叶片中NPR1表达量明显升高,与诱导抗病性密切相关。Induce peanut plants to spot disease resistance by spraying salicylic acid on leaves,pathogen culture filtrate,oxalic acid and other factors. Then determine the changes of host defense enzyme activity and NPR1 gene expression to explore the induced disease resistance and its mechanism. Results show that peanut can get systemic resistance by induction. Best results for 2. 0 mmol / L salicylic acid induced peanuts and the controlling efficacy was 35. 3%. The host defense enzyme activity was positively correlated with the intensity of induced disease resistance, but the induced disease resistance response changes of several defense enzymes varies greatly from the other. Realtime fluorescent quantitative PCR analysis showed that the tendency for 2. 0 mmol / L of SA induced NPR1 expression in peanut leaf volume increased significantly after treatment,which is closely related to the induction of disease resistance.

关 键 词：花生网斑病 诱导抗病性 水杨酸 寄主防御酶 NPR1基因 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]