HRAD17过表达促进辐射损伤诱导的A549细胞凋亡  

Overexpression of HRAD17 sensitizes A549 cells to radiation-induced apoptosis

作　　者：郭妍宏[1] 韩云[1] 朱应葆[1] 

机构地区：[1]北京大学第三医院中心实验室,100083

出　　处：《中华放射医学与防护杂志》2008年第2期-,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金

摘　　要：目的 探讨HRAD17对辐射损伤诱导细胞凋亡的影响及其机制.方法 将HRAD17转染人肺癌细胞A549经60Co γ射线照射后观察细胞凋亡变化.用Western blot检测凋亡相关基因表达水平,RT-PCR检测凋亡相关基因转录水平.结果 HRAD17转染可促进γ射线诱导的细胞凋亡.与对照组相比,HRAD17转染组p53蛋白Set 46磷酸化和p53 AIP1基因转录明显增强.结论 HRAD17可促进辐射损伤诱导的细胞凋亡,其部分机制可能是通过提高p53 AIP1的表达来实现的.Objective To study the effects of overexpression of HRAD17 on radiation-induced apoptosis of A549 cells.Methods Transfection of FLAC-tagged HRAD17 expression plasmid into human A549 cells was carried out and flow cytometry was employed tO observe the apoptotic effect.In addition,phosphorylation of p53 Ser46 was detected by Westem blot and the transcription of p53 AIP1 was measured by RT-PCR.Results Overexpression of HRAD17 gene significantly increased radiation-induced apoptosis of A549 cells.Further.Ser46 phosphorylation of P53 protein was increased and transcription of p53 AIP1 gene was elevated in cells overexpressing HRAD17.Conclusions HRAD17 protein can sensitize A549 cells to radiation-induced apoptosis in part by increasing p53 AIP1 expression.

关 键 词：细胞凋亡 HRAD17 P53 AIP1 

分 类 号：R686[医药卫生—骨科学]