胚外体腔穿刺术用于产前诊断的可行性研究  

A feasibility study of coelocentesis in prenatal diagnosis

作　　者：周凤珍[1] 李明[1] 高露露[1] 周霞平[1] 高素清[1] 李佳倪[1] 柯玮琳[1] 罗军[1] 

机构地区：[1]广东省深圳市第二人民医院妇产科,广东,深圳,518035

出　　处：《中国医师杂志》2008年第8期-,共3页Journal of Chinese Physician

基　　金：广东省科技基金重大项目面上课题

摘　　要：目的 探讨胚外体腔穿刺术的实施方法 及进行产前诊断的可行性研究.方法 孕6～12周要求人工流产的孕妇58例,在人工流产前行胚外体腔穿刺术抽取胚外体腔液,采用聚合酶链反应(PCR)技术检测Y染色体上SRY(sex-determining resion Y)基因,并以绒毛检测结果 作为对照标准.观察穿刺前后胎心率、囊内出血及血肿情况.结果 胚外体腔穿刺总成功率96.6%.穿刺均在5 min内完成,一次穿刺成功率孕6周～者为25.0%,孕7～10周者为93.2%,孕～12周者为50.0%.穿刺前后胎心率无明显变化(P＞0.05).胚外体腔细胞SRY基因检测的准确率为91.1%(51/56).其中30例女胎胚外体腔细胞检测结果 全部与绒毛相符;21例绒毛检测为男胎者,有5例胚外体腔细胞结果 不相符,男胎的准确率为80.8%(21/26).结论 胚外体腔穿刺术的最佳时间为孕7～10周,其操作简单易行,成功率高,安全可靠.Objective To explore the feasibility of coelocentesis in prenatal diagnosis.Methods Coelocentesis were applied on 58 women scheduled for at 6～12 weeks of gestation,and fetal heart rate before the procedure and 1,5,30 minutes afterwards and hemorrhage of amnionic cyst was observed.Y gene of sex-determining region was detected by polymerase chain reaction,and compared with chorionic villi sample.Results The total achievement ratio of coelocentesis was 96.6% of all cases in five minutes.The coelomic fluid was successfully aspirated at first attempt in 93.2% at 7～10 week's gestation,25.0% at 6～7weeks and 50.0% at 10 ~ 12 weeks.There was no significant difference in FHR between before coelocentesis and afterwards (P＞0.05).The detection accurate rate of SRY was 91.1%,all female fetus were matched with those observation by chorionic villi sampling,and only 5 male fetus were not matched,the accurate rate was 80.8%.Conclusion Coclocentesis has the advantage of simple procedure,safe and high feasibility.The optimal time of coclocentesis is in the 7th to 10th week of gestation.

关 键 词：产前诊断/方法 穿刺术/方法 

分 类 号：R714[医药卫生—妇产科学]