继发不育男性患者泌尿生殖道解脲脲原体基因分型鉴定分析  

Species identification and genotyping of ureaplasma in genitourinary of secondary infertifity of male

作　　者：谢小毛[1] 黄开淑[1] 李文华[1] 陈红娟[1] 张文权[1] 胡兰英[1] 

机构地区：[1]湖南省常德市第一人民医院生殖医学中心,湖南,常德,455003

出　　处：《中国医师杂志》2008年第12期-,共4页Journal of Chinese Physician

摘　　要：目的 了解继发不育男性患者泌尿生殖系解脲脲原体(UU)的感染状况.方法 对572例男性继发不育患者取尿道和前列腺分泌物标本进行培养,采用聚合酶链反应(PCR)和基因型分型技术,时其泌尿道生殖系UU进行生物变种和基因型分型鉴定,分析其结果.结果 572例男性继发不育患者,UU培养阳性278例(48.6%);PCR基因扩增检测UU-DNA阳性311例(54.4%).其中生物变种(biovar)1和生物变种2分别为212例(37.1%)和99例(17.3%);基因分型结果:生物变种1血清变种(serovar)1、3/14、6分别为71例(12.4%)、98例(17.2%)、43例(7.5%).生物变种2亚型(subtype)1、2、3分别为32例(5.6%)、51例(8.9%)、16例(2.8%).结论 UU感染是男性继发不育的重要危险因素,MBA多带抗原基因与16S-rRNA基因和尿素酶基因分型鉴定具有简便,快速,敏感,特异之优点.Objective To understand the infection of ureaplasma urealyticum in genitourinary of secondary infertility of male and ex-plore the relationship between the genotype of individual ureaplasma species and genitourinary infection of them . Methods Based on the multiple-banded antigen genes (MBA) of ureaplasma urealyticum, 10 pairs of oligonueleotide primers targeting the 5'ends of the MBA genes were designed to identify the MBA genes of U. parvum and U. ureaplasma by PCR-based genotyping system. The 10 pairs of oligonucleotide primers could distinguish the two biovars and 14 serovars of U. ureaplasma. Results A total of 278 (48.6%) positive ureaplasma culture were obtained from 572 patients attending our clinic of reproductive medical eenter. These methods were used to identify and genotype U. ureaplasma in 311 (54.4%) of 572 patients with genitourinary infection among them U. parvum (biovar 1) was detected in 37.1% and U. ureaplasma (biovar 2) in 17.8%. serovar 1 was in 12.4%, serovars3/14 in 17.1% serovar 6 in 7.5%; subtype 1 of biovar 2 was in 5.6%, subtype 2 in 8.9% and subtype 3 in 2.8%, respectively. Conclusion The PCR-based genotyping system will facilitate future stud-ies of relationship between individual Ureaplasma species or subtypes in genitourinary of secondary infertility of male. The methods described here are relatively rapid, practicable, and specific for the detection species identification and subtyping of Ureaplasma species.

关 键 词：不育 男(雄)性 解脲支原体 基因型 

分 类 号：R698.2[医药卫生—泌尿科学] R450[医药卫生—外科学]