兔病毒性出血症诊断方法研究进展  

Research Progress on Diagnosis Method of Rabbit Viral Hemorrhagic Disease

作　　者：李娇 王金良[1,2] 谢金文[1,2] 王艳[1,2] 王文秀[1,2] 沈志强[1,2] 

机构地区：[1]山东绿都生物科技有限公司,滨州256600 [2]山东省滨州畜牧兽医研究院,滨州256600

出　　处：《中国养兔》2013年第4期22-26,43,共6页Chinese Journal of Rabbit Farming

基　　金：山东省自主创新成果转化重大专项计划(2008ZHZX1A1103)

摘　　要：兔病毒性出血症是由兔病毒性出血症病毒引起的兔的一种烈性、毁灭性传染病。目前该病在世界范围内流行,给养兔业带来了巨大的经济损失。随着分子生物学与现代免疫学技术的日臻进步,兔病毒性出血症的各种检测方法不断改进完善。本文就近年来针对兔病毒性出血症的病毒分离、血凝和血凝抑制试验、RT-PCR、荧光定量RT-PCR、酶联免疫吸附试验、胶体金免疫层析技术等诊断方法的最新研究进展以及各种检测方法的优缺点进行全面论述,旨在为不同实验室寻找和建立适合自己的检测方法,及发展新型的检测方法提供参考,为兔瘟的诊断与防控提供合理的科学依据。The rabbit hemorrhagic disease(RHD) is a potent cause devastating infectious disease in rabbits caused by rabbit hemorrhagic disease virus(RHDV). The disease is prevalent in world wide,brought huge e conomic loss in rabbit industry. With the daily progress of techniques in molecular biology and modern immunology,various detection methods of rabbit viral hemorrhagic disease were continuously improved and perfected. This paper discussed the advantages and disadvantages of the new research method of diagnosis in recent years for rabbit viral isolation,hemagglutination and hemagglutination inhibition test,RT-PCR,fluorescence quantitative RT-PCR,enzyme-linked immunosorbent assay,colloidal gold immune chromatography technology progress and a variety of detection methods,aimed at finding and to establish detection methods for different laboratory,to provide reference for the development of new detection method,to provide a reasonable scientific basis for diagnosis and prevention of rabbit plague.

关 键 词：兔病毒性出血症 诊断方法 研究进展 

分 类 号：S858.291[农业科学—临床兽医学]