F11R基因多态性及其与家兔非特异性消化道紊乱易感性的关联分析  

Association Analysis of F11R Gene SNP and Non-specific Digestive Disorder in Rabbits

作　　者：李春梅[1] 刘文超[1] 杨智娟[1] 贾伟[1] 陈仕毅[1] 赖松家[1] 

机构地区：[1]四川农业大学成都校区动物遗传育种研究所,成都611130

出　　处：《中国养兔》2013年第8期4-7,11,共5页Chinese Journal of Rabbit Farming

基　　金：国家兔产业技术体系建设(CARS-44-A-2)

摘　　要：采用PCR产物直接测序法寻找家兔F11R基因变异位点,利用PCR-SSCP分型技术对新西兰兔共257个(患病组:n=129,健康对照组:n=128)个体进行基因分型及case-control关联分析。结果发现家兔F11R基因内含子2上存在一个SNP位点(c.178+177 C>T),c.178+177 C和c.178+177 T在case组和control组中平均基因频率为分别为0.5175和0.4825,三种基因型CC、CT和TT的频率分别为0.2724、0.4903和0.2373。该位点在case和control的整个群体中表现出中度多态(0.25<PIC<0.50)。case-control关联分析表明,F11R基因c.178+177 C>T位点与家兔非特异性消化道紊乱不相关(P>0.05)。A total of 257 individuals(case: n = 129, control: n = 128) from New Zealand rabbit were chosen for the experiment to analyze the genetic characteristic of F11R gene and case-control association study. Direct sequencing was applied to detect the SNP loci of F11R gene,and PCR-SSCP technology was conducted to genotype F11R gene. A lot of statistical analysis was applied to calculate the gene frequencies and genotype frequencies. Case-control study was applied to analysis the association between F11R gene SNP and non-specific digestive disorder. The result showed: One SNP(c.178+177 C > T) was detected in the intron2 of rabbit F11R gene. The allele frequencies of c.178+177 C and c.178+177 T in case and control group were 0.5175 and 0.4825, respectively. The frequencies of genotypes CC、CT and TT were 0.2724, 0.4902 and 0.2373. The genetic polymorphism analysis showed that the polymorphism of c.178+177 C>T in New Zealand rabbit breeds showed moderate polymorphism(0.25 <PIC <0.50). But the case-control association study indicated that there was no association between F11R SNP and non-specific digestive disorder in rabbit(P>0.05).

关 键 词：家兔 F11R 非特异性消化道紊乱 SNP SSCP Case-control关联分析 

分 类 号：S858.291[农业科学—临床兽医学]